变转速策略调控灵芝菌丝体发酵高产三萜  

Enhancement of ganoderic acid production in the two‐stage fermentation by using variable speed control strategy

作　　者：赵娜[1,2] 贾薇[1] 冯杰[1] 冯娜[1] 唐传红[1] 刘艳芳[1] 杨焱[1] 唐庆九[1] 张劲松[1] 

机构地区：[1]国家食用菌工程技术研究中心农业部南方食用菌资源利用重点实验室上海市农业遗传育种重点开放实验室上海市农业科学院食用菌研究所,上海201403 [2]上海海洋大学食品学院,上海201306

出　　处：《菌物学报》2015年第1期131-138,共8页Mycosystema

摘　　要：灵芝发酵过程中,采用变转速调控策略,对振荡发酵阶段进行优化,以期达到高产三萜的目的。振荡阶段最佳条件为转速由150r/min变为100r/min,该策略与液体静置培养相结合,最终菌丝体三萜产量高达678.0g/L,比优化前提高了21%。振荡发酵阶段的变转速策略有效地提高了三萜的产量。By using key stage‐speed control strategy, the two‐stage culture process of Ganoderma lingzhi was optimized in order to obtain hyper‐production of total GA content. The process of variable speed from 150r/min to 100r/min, as the optimal condition in oscillation culture phase, was combined with liquid static culture. In process of the novel two‐stage culture, the total Ganoderic acids production（GA） of G. lingzhi was improved to 678.0g/L which was 21% higher than the control. It is effective to improve total GA production by using stage‐speed control strategy in oscillation culture stage.

关 键 词：灵芝 两阶段静置培养 变转速 三萜 

分 类 号：S567.31[农业科学—中草药栽培]