固相萃取/液相色谱-串联质谱法测定鱼塘水中双甲脒及其代谢产物  

Detection of Amitraz and Its Metabolites in Pond-water Using SPE / LC-MS / MS

作　　者：郭浩[1] 郭东东[1] 李恒[1] 刘文文[1] 丁静[1] 郑经[1] 王俊伟[1] 

机构地区：[1]重庆市公安局物证鉴定中心,重庆400021

出　　处：《分析测试学报》2014年第12期1416-1420,共5页Journal of Instrumental Analysis

基　　金：重庆市公安局科技攻关计划项目(G2012-01)

摘　　要：建立了鱼塘水中双甲脒及其代谢产物的液相色谱-串联质谱检测方法.采用固相萃取前处理,考察了不同的固相萃取柱和洗脱溶剂对检测结果的影响,优化了固相萃取条件.结果表明,在优化条件下,4种目标物在20～2 000 ng/L范围内线性关系良好（r为0.997 5 ～0.999 1）,该方法对双甲脒及其代谢产物的检出限为0.4～1.0 ng/L.鱼塘水中双甲脒及其代谢产物的回收率为69.7％～ 91.3％,方法具有良好的灵敏度、重现性、稳定性和专属性,可满足日常的分析要求.A method was developed for the quantitative determination of amitraz and its metabolites in pond-water by solid phase extraction and liquid chromatography-tandem mass spectrometry.The extraction and separation efficiencies of analytes were investigated using different organic solvents and SPE columns.In the optimal conditions,the calibration curves were linear in the range of 20-2 000 ng/L.The limits of detection for amitraz and its metabolites were in the range of 0.4-1.0 ng/L.The recoveries of these analytes were in the range of 69.7％-91.3％.This method exhibited high sensitivity,good repeatability,long-time stability and specificity,and could be used to detect amitraz and its metabolites in pond-water.

关 键 词：双甲脒 单甲脒 2 4-二甲基苯基甲酰胺 2 4-二甲基苯胺 固相萃取 鱼塘水 液相色谱-串联质谱 

分 类 号：O657.63[理学—分析化学] F767.2[理学—化学]