迷迭香叶中鼠尾草酸的提取工艺研究  

Study on the Extraction Process for Carnosic Acid from Rosmarinus officinalis Linn

作　　者：魏静[1] 王尚文[2] 于浩飞[1] 赵荣华[3,4] 王扣[1] 张荣平[1,4] 

机构地区：[1]昆明医科大学药学院暨云南天然药物药理重点实验室,云南昆明650500 [2]昆明医科大学法医学院,云南昆明650500 [3]云南中医学院中药学院,云南昆明650500 [4]南药协同创新中心,云南昆明650500

出　　处：《昆明医科大学学报》2015年第1期58-61,共4页Journal of Kunming Medical University

基　　金：云南省天然药物药理重点实验室开放研究基金资助项目(2013G013)

摘　　要：目的优选迷迭香叶中鼠尾草酸的提取工艺.方法以鼠尾草酸提取率为定量指标,确定最佳提取溶剂和提取方法.在单因素试验基础上,采用正交试验考察提取时间、料液比和提取次数对鼠尾草酸提取率的影响.结果采用乙酸乙酯为溶剂,超声提取,料液比1:14,提取4次,每次75 min,在此条件下鼠尾草酸平均提取率达2.479%.结论优选的鼠尾草酸提取工艺稳定,重复性好.Objective To optimize the extraction process for carnosic acid from leaves of Rosmarlnus officinalis Linn. Methods With the extraction rate of carnosic acid as quantitative index, the best extraction solvent and extraction method were identified by single factor experiments. And then, the effects of extraction time, solid-liquid ratio and extraction times on extraction rate of carnosic acid were investigated Results The optimal extraction technology was as follows： ethyl acetate extracting with material by orthogonal tests. and solvent ratio of 1： 14, ultrasonic extraction for 4 times, wand 75 min each time. Under these conditions, the average extraction rate of camosic acid was up to 2.479%. Conclusion The optimal extraction process for carnosic acid from R. officinalis was reproducible and stable.

关 键 词：鼠尾草酸 提取工艺 迷迭香 

分 类 号：R284.2[医药卫生—中药学]