塔克拉马干沙漠存在超耐β-内酰胺环抗生素微生物的证据及对一个新物种的生物化学特性描述（英文）  

Evidence of The Presence of Bacteria Highly Resistant to β-Lactam Antibiotics in Taklimakan Desert and Biochemical Characterization of Paramesorhizobium deserti gen. nov., sp. nov.

作　　者：罗雪松[1] 潘云帆[2] 石晶晶[3] 代俊[4] 侯莎[3] 方呈祥[3] 彭芳[3] 

机构地区：[1]华中农业大学农业微生物国家重点实验室,资源与环境学院,武汉430070 [2]中国地质大学环境学院,武汉430074 [3]武汉大学生命科学学院,武汉430072 [4]湖北工业大学生物工程学院,武汉430068

出　　处：《生物化学与生物物理进展》2015年第1期56-64,共9页Progress In Biochemistry and Biophysics

基　　金：supported by a grant from The Fundamental Research Funds for The Central Universities(2662014QC022)~~

摘　　要：在对塔克拉玛干沙漠土著微生物进行研究过程中,发现10株细菌能够在含有1 000 mg/L氨苄青霉素的0.1×TSB平板上生长.这些微生物具有广泛的β-内酰氨类抗生素耐受性.它们均属于变形杆菌纲,其中5个菌株鉴定为条件致病菌Stenotrophomonas matltophila,还有4个菌株与Mesorhizobium amorphae亲缘关系很近.有趣的是,菌株A-3-E T与已知物种同源性低,通过基于生物化学方法的多相分类学研究,我们将其确定为一个新属,并命名为Paramesorhizobium desertii gen.nov.,sp.nov.,发现高浓度的β-内酰胺类抗生素,如1000 mg/L的唑啉头孢菌素或250 mg/L的头孢氨噻肟,依然无法抑制其生长.此外,该菌株还能耐受所测试28种抗生素中的17种,塔克拉玛干沙漠可能是一个新的β-内酰胺环抗生素耐药细菌的自然资源库.During the surveying of indigenous bacterial diversity in Taklimakan Desert of Xinjiang of China, we obtained 10 isolates,which possess a capacity to grow on 0.1 ×Tryptic Soy Broth agar supplemented with 1000 mg/L ampicillin. These bacteria display extensive resistances to different β-lactam antibiotics. All of these isolates belong to Proteobacteria. Five of them were identified as the human opportunistic pathogen Stenotrophomonas matltophila. Four strains were closely related to Mesorhizobium amorphae. It is interesting to see that one isolate A-3-E ^T showed low 16 S r RNA gene sequence similarity（ 〈96.8%） to those of the recognized species.The results of polyphasic taxonomy showed the strain represents a novel species of the new genus, for which the name Paramesorhizobium desertii gen. nov., sp. nov. is proposed. Moreover, it was found that high concentrations of β-lactam antibiotics are not able to inhibit the growth of strain A-3-E T. The novel bacterium grows well in the media containing 1000 mg/L of cefazolin or250 mg/L of cefotaxime. The strain also resists 17 of the 28 tested antibiotics. We just presented a case that the Taklimakan Desert is a natural reservoir of novel β-Lactam antibiotic resistant bacteria.

关 键 词：Β-内酰胺类抗生素 抗药细菌 塔克拉马干沙漠 

分 类 号：Q939.1[生物学—微生物学]