人管家基因启动子序列中的组合转录调控元件分析  

Analysis of Combinatorial Transcriptional Regulatory Elements in Promoters of Human Housekeeping Genes

作　　者：李慧敏[1,2] 石玉[3] 杨志刚[4] 江绍萍[1] 

机构地区：[1]云南民族大学数学与计算机科学学院,昆明650500 [2]云南大学生物资源保护和利用重点实验室,昆明650091 [3]郑州师范学院数学与统计学院,郑州450044 [4]四川大学计算机学院,成都610065

出　　处：《生物化学与生物物理进展》2015年第1期73-81,共9页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(31360277,31460297和11461083);云南省教育厅科学研究项目(2013Z042);河南省教育厅科学技术项目(14A110020);云南大学理工类科研基金项目(2012CG016)资助~~

摘　　要：研究表明,第一内含子可能参与基因转录调控.利用统计方法提取人管家基因上游至第一内含子序列中潜在的组合转录调控模体,分析模体间的距离、区域分布等特征,探讨内含子参与基因转录调控的可能性及其参与方式.在管家基因中共获得960对潜在转录调控模体对,其中57%与实验已知的具有转录相互作用的因子对吻合,共涉及12组因子对.分析发现,绝大多数模体对(>80%)偏向于上游区域及"上游-内含子"区域,进一步支持了内含子参与基因转录调控的假设,并据此推测内含子与上游序列之间具有转录协同作用,模体在基因转录起始位点(TSS)附近较为集中,模体对的两个模体之间距离较近,60%左右距离在200 bp以内,特别地,65%的模体对特征距离在100 bp以内,短距离间隔有利于转录因子间的协同作用.这些结果将有助于对人基因转录调控机制及内含子功能的深入认识.A lot of researches suggest that there are transcriptional regulatory elements in the first introns of genes. However, the ways about introns participating in transcriptional regulation are not still understood. In this paper, we extracted combinatorial transcriptional regulatory motifs in regions from upstream sequences to the first introns in human housekeeping（HK） genes by statistical methods, analyzed the characteristics of distances and locations of motifs, and discussed the potential and ways of introns involving in transcriptional regulation. 960 potential transcriptional regulatory motif pairs are extracted in HK genes, and 57% of them are in accordance with12 known interacting transcription factor pairs. The location preferences of the over-represented motif pairs have been studied. The results show that about 80% and 90% of the motif pairs in HK genes occur in upstream-upstream and upstream-intron regions, respectively. This results further support the conjecture of the introns could participate in transcriptional regulation of genes, and it was speculated that there are transcriptional synergism between introns and upstream promoter. Extracting motifs are mainly focus on vicinity of transcriptional start sites（TSSs）, and the space distance between two motifs in one motif pairs is short. The preference analyses on distance of the motif pairs show that 60% of the motif pairs in HK genes are shorter than 200 bp, and 65% of characteristic distances are within 100 bp. This is conductive for synergism of transcription factors. These results will be helpful for understanding the mechanisms of the transcriptional regulation for HK genes in human.

关 键 词：人管家基因 组合转录调控 模体对 内含子 区域偏好 距离分布 

分 类 号：Q6[生物学—生物物理学] Q7