Effects of senescence on the expression of BDNF and TrkB receptor in the lateral geniculate nucleus of cats  

作　　者：Chuan-Wang TONG Zi-Lu WANG Peng LI Hui ZHU Cui-Yun CHEN Tian-Miao HUA 

机构地区：[1]College of Life Science, Anhui Normal University, Wuhu 241000, China [2]College of Biological Engineering, Wuhu Institute of Technology, Wuhu 241000, China

出　　处：《Zoological Research》2015年第1期48-53,共6页动物学研究（英文）

基　　金：This study was supported by the National Natural Science Foundation of China （31171082）; the Key Project of Natural Science Research of the Education Bureau of Anhui Province （KJ2014A284）; the Foundation of Key Laboratories of Anhui Province and Key Laboratories of Universities of Anhui Province

摘　　要：To explore the neural mechanisms mediating agingrelated visual function declines, we compared the expressions of brain-derived neurotrophic factor（BDNF） and its high affinity receptor-tyrosine kinase B（Trk B） between young and old adult cats. Nissl staining was used to display neurons in each layer of the lateral geniculate nucleus（LGN）. The BDNF- and Trk B receptor-immunoreactive neurons were labeled immunohistochemically, observed under optical microscope and photographed. Their neuronal density and immunoreactive intensity were measured. Results showed that the mean density of the Nissl stained neurons in each LGN layer were comparable between old and young adult cats, and their BDNF and Trk B proteins were widely expressed in all LGN layers. However, compared with young adult cats, both the density and optical absorbance intensity of BDNF- and Trk B-immunoreactive cells in each LGN layer in old cats were significantly decreased. These findings indicate that the decreased expressions of BDNF and Trk B proteins in the LGN may be an important factor inducing the compromised inhibition in the central visual nucleus and the functional visual decline in senescent individuals.To explore the neural mechanisms mediating agingrelated visual function declines, we compared the expressions of brain-derived neurotrophic factor（BDNF） and its high affinity receptor-tyrosine kinase B（Trk B） between young and old adult cats. Nissl staining was used to display neurons in each layer of the lateral geniculate nucleus（LGN）. The BDNF- and Trk B receptor-immunoreactive neurons were labeled immunohistochemically, observed under optical microscope and photographed. Their neuronal density and immunoreactive intensity were measured. Results showed that the mean density of the Nissl stained neurons in each LGN layer were comparable between old and young adult cats, and their BDNF and Trk B proteins were widely expressed in all LGN layers. However, compared with young adult cats, both the density and optical absorbance intensity of BDNF- and Trk B-immunoreactive cells in each LGN layer in old cats were significantly decreased. These findings indicate that the decreased expressions of BDNF and Trk B proteins in the LGN may be an important factor inducing the compromised inhibition in the central visual nucleus and the functional visual decline in senescent individuals.

关 键 词：nucleus neuronal neurotrophic stained labeled comparable young absorbance TrkB senescence 

分 类 号：Q95-33[生物学—动物学]