聚己内酯静电纺丝纳米纤维支架用于小鼠诱导多能干细胞培养  

Electrospun Poly(ε-caprolactone) Nanofibrous Scaffolds for Mouse Induced Pluripotent Stem Cell Cultivation

作　　者：陈焱[1,2] 曾迪[1] 丁璐[1] 李晓莉[1] 谢江徽[2] 郑强荪[1] 

机构地区：[1]第四军医大学唐都医院心内科,西安710038 [2]解放军第401医院急诊科,山东青岛266071

出　　处：《中国生物化学与分子生物学报》2015年第1期72-79,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.31271039;No.31400832)~~

摘　　要：干细胞联合生物支架材料体外构建功能性组织与器官,成为当前组织再生研究的重要策略,而探求具有良好生物相容性的支架材料是其关键.本研究采用扫描电镜、噻唑蓝(MTT)法、荧光显微染色等方法检测小鼠诱导多能干细胞(murine induced pluripotent stem cells,mi PSCs)在聚己内酯(polyε-caprolactone,PCL)静电纺丝纳米纤维支架上的粘附、增殖等生物学特性,探究聚己内酯纳米纤维支架与mi PSCs的生物相容性.结果显示,mi PSC在PCL纳米纤维支架上具有良好粘附性并呈集落样生长,其增殖能力及干性标记物(Oct4-GFP+)的表达均不亚于标准对照组;扫描电镜显示,mi PSC在PCL纳米纤维支架材料上呈现出绒毛状突起的表面结构.上述结果表明,PCL纳米纤维支架可促进mi PSCs的粘附、自我增殖以及干性维持,两者具有良好的生物相容性,为下一步联合生物支架材料与干细胞构建功能性组织奠定了基础.In vitro engineering of functional tissue and organ with stem cells and biomaterial scaffold become a significant strategy for tissue regeneration at present,whereas exploring scaffolds with good biocompatibility is critical. To evaluate the biocompatibility of murine induced pluripotent stem cell（mi PSCs） on the poly ε-caprolactone（PCL） nanofibrous scaffold,in this study,the biological behavior of mi PSCs on the PCL nanofibrous scaffold,such as adhesion and proliferation were detected using scanning electron microscopy,MTT assay and fluorescence staining. The results showed that mi PSCs adhered well and grew in colony on the PCL nanofibrous scaffold,similar to Oct4-GFP＋mi PSCs cultured with MEF feeder-layers in tissue culture plates. Moreover,the proliferation and pluripotency（expression of Oct4-GFP＋） of mi PSCs in scaffold group were as good as control group. Scanning electron micrographs revealed that the surface topography of mi PSCs grown on the PCL nanofibrous scaffold was characterized by a cilium-like structure. These results demonstrated that the electrospun PCL nanofibrous scaffold could promote the adhension, self-renewal and pluripotency maintenance of mi PSCs, and had a good biocompatibility with Oct4-GFP＋mi PSCs,which laid foundations for further research on functional tissueengineering with stem cells and biomaterial scaffold.

关 键 词：诱导多能干细胞 静电纺丝 聚己内酯 组织工程 

分 类 号：Q24[生物学—细胞生物学] TB324[一般工业技术—材料科学与工程]