不同来源前列腺基质细胞对前列腺癌细胞IGF-1和p-AKT表达的影响  

Stromal cells from different sources influence the expression of IGF-1 and p-AKT in prostate cancer cells

作　　者：倪少滨[1] 刘迪[1] 赵忠山[1] 麻立[1] 焦治兴[1] 

机构地区：[1]哈尔滨医科大学附属第一医院泌尿外科,黑龙江哈尔滨150001

出　　处：《现代泌尿外科杂志》2015年第1期52-54,共3页Journal of Modern Urology

基　　金：黑龙江省自然科学基金(No.D201080)

摘　　要：目的探讨胰岛素样生长因子在前列腺癌肿瘤-基质交互作用中的地位及其作用机制。方法将前列腺癌旁基质细胞和良性前列腺基质细胞分别与前列腺癌细胞系PC-3细胞分隔共培养(并设空白对照),之后检测前列腺癌细胞中IGF-1 mRNA(半定量RT-PCR)和p-AKT蛋白表达(Western blot)。结果与空白对照组相比较,癌旁基质细胞共培养组IGF-1mRNA表达有所上调、p-AKT蛋白表达有所上调,但无统计学意义(P>0.05);良性基质细胞共培养组IGF-1mRNA和p-AKT蛋白表达显著下调(P<0.05)。相关性分析显示各组前列腺癌细胞IGF-1mRNA与p-AKT蛋白表达呈正相关。结论良性前列腺基质细胞通过水溶性因子抑制前列腺癌细胞中IGF-1及其下游因子表达,前列腺癌旁基质细胞则丧失了该抑制作用。Objective To explore the role of insulin-like growth factor （IGF） in tumor-stromal cell interactions of prostate cancer. Methods PC-3 cells were seeded in TRANSWELL plate and interacted with different effector cells including benign and cancer-associated prostate stromal cells. Semi-quantitative RT-PCR was used to detect the expression of 1GF-1 mR- NA; Western blot was used to detect the expression of p-AKT. Results Compared with the control group, the expression of IGF-1 mRNA and p-AKT in PC-3 cells cocultured with benign prostate stromal cells were decreased （P〈0. 05）, while there were no significant changes in the expression of IGF-1 mRNA and p-AKT in PC-3 ceils cocultured with cancer-associated pros- tate stromal cells. The expression of IGF-1 mRNA was positively correlated with the expression of p-AKT （r = 0. 723, P〈 0. 05）. Conclusion Benign prostate stromal cells can suppress the expression of IGF-1 mRNA and its downstream protein factor in PC-3 cells, while cancer-associated prostate stromal cells do not have this effect.

关 键 词：前列腺癌 胰岛素样生长因子 基质细胞 

分 类 号：R697.3[医药卫生—泌尿科学]