原发性高血压患者外周血单个核细胞中MMP-9和TIMP-1的表达及意义  

Expressions and significance of MMP-9 and TIMP-1 in peripheral blood mononuclear cells in patients with essential hypertension

作　　者：王书乐[1] 周春刚[1] 苏伟[1] 张志斌[1] 

机构地区：[1]无锡市中医医院心血管病实验室,江苏省214000

出　　处：《江苏医药》2015年第1期24-26,共3页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK2008117)

摘　　要：目的探讨原发性高血压(EH)患者外周血单个核细胞中基质金属蛋白酶9(MMP-9)和基质金属蛋白酶组织抑制因子1(TIMP-1)的表达及意义。方法 EH患者94例(EH组)分为单纯EH(A组,20例)和合并颈动脉粥样硬化(B组,74例),另选健康人60例为对照(C组),采用实时荧光定量PCR检测外周血单个核细胞中MMP-9 mRNA、TIMP-1 mRNA水平。结果EH组MMP-9mRNA水平和MMP-9mRNA/TIMP-1mRNA比值为1.264±0.135和2.313±0.335,B组MMP-9mRNA水平和MMP-9mRNA/TIMP-1mRNA比值为1.353±0.159和2.549±0.413,均高于C组的0.796±0.078和1.132±0.180(P<0.05)。A组的上述指标与C组无明显差异(P>0.05)。结论 EH患者外周血单个核细胞中MMP-9mRNA和TIMP-1mRNA可作为EH相关血管活性异常的间接标志物。Objective To explore the expressions and significance of matrix metalloproteinase 9（MMP-9）and tissue inhibitor of matrix metalloproteinase 1（TIMP-1）in peripheral blood mononuclear cells（PBMC）in the patients with essential hypertension（EH）.Methods A total of 94 patients with EH（group EH）was divided into two groups of A（without atherosclerosis,20cases）and B（with atherosclerosis,74cases）.Sixty healthy people were taken as the controls（group C）.The Levels of MMP-9mRNA and TIMP-1mRNA in PBMC were detected by RQ-PCR.Results MMP-9mRNA expression and the ratio of MMP-9mRNA to TIMP-1mRNA were 1.264±0.135 and 2.313±0.335 in group EH,and 1.353±0.159 and 2.549±0.413 in group B,which were all higher than0.796±0.078 and 1.132±0.180 in group C（P〈0.05）,but were not significantly difference between groups of A and C（P〉0.05）.Conclusion MMP-9 mRNA and TIMP-1 mRNA of PBMC in EH patients may be served as the indirect markers for EH-related abnormal activity.

关 键 词：原发性高血压 外周血单个核细胞 基质金属蛋白酶9 基质金属蛋白酶组织抑制因子1 

分 类 号：R544[医药卫生—心血管疾病]