煤工尘肺患者血清TGF-β1和IL-17A水平及其与肺功能关系  

The relationship between serum levels of TGF-β1,IL-17A and lung function in patients with coal worker's pneumoconiosis

作　　者：袁宝军[1] 李超[1] 王冬梅[1] 邹吉敏[1] 马毅华 朱秀萍 

机构地区：[1]开滦总医院检验科 [2]开滦职业病防治院,河北唐山063000

出　　处：《中国实验诊断学》2014年第6期906-909,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨血清转化生长因子β1(TGF-β1)和白细胞介素-17A(IL-17A)水平在煤工尘肺(CWP)中的变化及其与肺功能关系。方法采用双抗体夹心ELISA法检测118例CWP患者(CWP组)、61例具有与CWP组相同接尘条件的健康井下接尘矿工(接尘对照组)及53例井上健康查体人员(正常对照组)血清TGF-β1和IL-17A水平;用肺功能机检测用力肺活量(FVC%)、一秒钟用力呼气量(FEV1%)、用力呼气1秒率(FEV1/FVC%)、最大呼气中期流速(MMEF75/25%)4项肺功能指标。结果与正常对照组比较,CWP组TGF-β1和IL-17A水平明显升高,差异具有统计学意义(P<0.05和P<0.01);CWP组IL-17A水平明显高于接尘对照组(P<0.01),而4项肺功能指标均明显降低,差异具有统计学意义(P<0.05和P<0.01)。CWP并发慢性阻塞性肺疾病组TGF-β1水平明显低于单纯CWP组(P<0.01)。CWP组TGF-β1与FEV1/FVC(%)呈负相关(r=-0.427,P<0.05)。结论 CWP患者血清TGF-β1水平可能与疾病严重程度有关,并间接反映肺功能;检测IL-17A水平可能对协助诊断CWP有一定参考价值;TGF-β1和IL-17A在CWP发生发展中可能各自发挥其作用。Objective To explore the relation of serum transforming growth factorβ1（TGF-β1）,interleukin-17A（IL-17A）levels and lung function in patients with coal worker’s pneumoconiosis （CWP）.Methods Methods The levels of serum TGF-β1 and IL-17A were determined in 118 CWP patients （CWP group）,61 miners （dust exposed control group）and 53 healthy controls by sandwich ELISA;the forced vital capacity （FVC%）,forced expiratory volume in one second （FEV1%）,（FEV1/FVC%）and maximal mid-expiratory flow （MMEF75/25%）were determined by special lung function machine.Results Compared with healthy control group,the levels of TGF-β1 and IL-17A were signifi-cantly higher in CWP （P 〈0.05,P 〈0.01）,IL-17A in CWP were significantly higher than that of dust exposed control group （P 〈0.01）.Four parameters of lung function in CWP were lower distinctly than that of dust exposed control group（P 〈0.05,P 〈0.01）.TGF-β1 levels were significantly lower in CWP with chronic obstructive pulmonary disease than that of simple CWP（P〈0.01）.In CWP,TGF-β1 were negatively correlated with FEV1/FVC（%）（r=-0.427,P〈0.05）.Conclusion The levels of serum TGF-β1 in CWP may be related to disease severity,and reflect the lung func-tion indirectly;the detection of seurm IL-17A levels may be valuable for diagnosis of CWP;TGF-β1 and IL-17A may play a role respectively in the occurrence and development of CWP.

关 键 词：煤工尘肺 转化生长因子Β1 白细胞介素-17A 肺功能 

分 类 号：R135.2[医药卫生—劳动卫生]