PAE-MHT法检测铜绿假单胞菌金属β-内酰胺酶的临床适用性的评估  

Evaluation of Pseudomonas aeruginosa-Modified Hodge Test for detection of metallo-β-lactamase-producing Pseudomonas aeruginosa

作　　者：谢国艳[1] 高志生[1] 许俊[1] 秦云[1] 蔡晓云[1] 

机构地区：[1]上海交通大学医学院附属新华医院崇明分院,上海202150

出　　处：《中国实验诊断学》2014年第6期982-985,共4页Chinese Journal of Laboratory Diagnosis

基　　金：上海市崇明社会发展基金(ck201136)

摘　　要：目的评估铜绿假单胞菌-改良Hodge(PAE-MHT)法对铜绿假单胞菌(PAE)金属β-内酰胺酶(MBLs)检测的临床适用性。方法收集82株临床分离的耐亚胺培南的PAE,通过双纸片协同法、E-test法及PAE-MHT法作为MBLs表型初筛试验,并对几种方法进行比较。聚合酶链反应(PCR)法检测IMP型和VIM型MBLs基因并测序。结果 PAE-MHT法的敏感性、特异性和准确率分别为84.6%,97.2%,97.6%.PAE-MHT法和双纸片协同法、Etest法有非常好的一致性。结论 PAE-MHT法简便、快捷,不失为临床快速筛查非发酵菌产MBLs的一种新方法。Objective To Evaluate Pseudomonas aeruginosa-Modified Hodge Test（PAE-MHT）for detection of metallo-β-lactamase-producing Pseudomonas aeruginosa （PAE）in the clinical microbiology laboratory.Methods A total of 82 imipenem-resistant PAE clinical isolates was dectected to metallo-β-lactamase（MBLs）by double disk synergy test、E-test and PAE-MHT,and compared with the positive rates of three methods.The IMP、VIM genes were detected by polymerase chain reaction（PCR）.Results The sensitivity,specificity and accuracy rate of PAE-MHT is 84.6%, 97.2%,97.6%.Three methods had a good consistency.Conclusion PAE-MHT is a simple,quick test on Pseudomonas for daily use in clinical laboratories.

关 键 词：铜绿假单胞菌 金属Β-内酰胺酶 双纸片协同试验 评估 

分 类 号：R446.5[医药卫生—诊断学]