尘肺患者血清中基质金属蛋白酶9和19的表达  

Serum levels of matrix metalloproteinase 9 and 19 in the patients with pneumoconiosis

作　　者：吕玉民 王平 郭艳[2] 李峰[3] 陶雅[3] 刘康栋[3] 赵凤玲 李时恩[4] 

机构地区：[1]河南省职业病防治研究院,郑州450052 [2]郑州市第一人民医院 [3]郑州大学基础医学院 [4]郑州大学公共卫生学院劳动卫生教研室

出　　处：《中华劳动卫生职业病杂志》2015年第1期29-32,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家重点临床专科建设项目（2011-17）;国家重大基础研究计划（2012CB933304）;河南省重点科技攻关计划项目（142102310086）;河南省医学科技攻关项目（201003146）

摘　　要：目的 检测尘肺病患者外周血清中基质金属蛋白酶（MMP）9和19的表达水平,探讨其作为尘肺病相关生物指标的可行性.方法 选择98例男性尘肺病患者（Ⅰ期49例,Ⅱ期36例,Ⅲ期13例）,其中矽肺41例,煤工尘肺57例.98例男性健康体检者作为对照.空腹情况下采集3 ml外周静脉血,分离血清,用酶联免疫吸附法测定样本血清中MMP9和MMP19水平.结果 矽肺组血清中MMP9和MMP19表达均低于对照组,差异有统计学意义（P＜0.05）;煤工尘肺组血清中MMP9和MMP19表达均低于对照组,差异有统计学意义（P＜0.05）;矽肺组血清中MMP19表达水平高于煤工尘肺组,差异有统计学意义（P＜0.05）.接尘工龄≤7年患者血清中MMP19表达水平高于接尘工龄＞20年的患者,差异有统计学意义（P＜0.05）.不同肺功能损伤患者血清中MMP9和MMP19表达,差异无统计学意义（P＞0.05）.尘肺病组与健康对照组血清中MMP9和MMP19表达均呈明显正相关关系（r=0.235,P＜0.05）;血清中MMP9表达与尘肺分期呈负相关关系（P＜0.05）.结论 尘肺病患者血清中MMP9和MMP19表达变化有作为尘肺病相关生物标志物的潜能.Objective To measure peripheral serum levels of matrix metalloproteinase 9 （MMP9） and matrix metalloproteinase 19 （MMP19） in patients with pneumoconiosis,and to investigate their feasibility as potential biomarkers for pneumoconiosis.Methods Ninety-eight male patients with pneumoconiosis （49patients in phase Ⅰ,36 patients in phase Ⅱ,and 13 patients in phase Ⅲ） were enrolled as subjects,which included 41 patients with silicosis and 57 patients with coal workers＆#39; pneumoconiosis.Ninety-eight healthy male physical examinees were used as controls.A fasting blood sample （3 ml） was collected from the peripheral venous blood of each patient or control,and the serum was separated from the blood sample.The expression levels of MMP9 and MMP19 in serum were measured by enzyme-linked immunosorbent assay.Results Serum levels of MMP9 and MMP19 in patients with silicosis or coal workers＆#39; pneumoconiosis were significantly lower than those in the control group （P〈0.05）.Serum levels of MMP19 in patients with silicosis were significantly higher than those in patients with coal workers＆#39; pneumoconiosis （P〈0.05）.Serum levels of MMP19 in patients exposed to dust for less than 7 years were significantly higher than those in patients exposed to dust for more than 20 years （P〈0.05）.There were no significant differences in serum levels of MMP9 and MMP19 between patients with different levels of pulmonary function impairment （P〉0.05）.Serum expression levels of MMP9 and MMP19 were positively correlated with each other in both patients with pneumoconiosis and those in the control group （P〈0.05）.The serum expression level of MMP9 was negatively correlated with the stage of pneumoconiosis （P〈0.05）.Conclusion Serum MMP9 and MMP19 may be used as potential biomarkers for pneumoconiosis.

关 键 词：尘肺 血清 基质金属蛋白酶 

分 类 号：R135.2[医药卫生—劳动卫生]