胃泌素释放肽前体双抗体夹心酶联免疫吸附法的建立及其应用  

Establishment of double antibody sandwich ELISA for pro-gastrin releasing peptide and its application

作　　者：楚振宇[1,2] 周小林[1] 薛振伟[1] 崔梅萍[1] 蔺苏琴 李瑞华 

机构地区：[1]中国辐射防护研究院放射医学与环境医学研究所,太原030006 [2]武警总医院 [3]太原肿瘤医院肿瘤内科,太原030006

出　　处：《医学研究生学报》2015年第1期70-73,共4页Journal of Medical Postgraduates

摘　　要：目的胃泌素释放肽前体(pro-gastrin releasing peptide,PGRP)作为小细胞肺癌肿瘤标志物的应用价值已成为近年来的研究热点,文中建立新型双抗体夹心酶联免疫吸附法(enzyme-linked immune sorbent assay,ELISA)方法检测患者血清PGRP抗原浓度。方法通过人工合成PGRP抗原决定簇对本实验室自行研制的单克隆抗体进行筛选;采用改良过碘酸钠法对筛选到的单克隆抗体进行辣根过氧化物酶标记,建立PGRP双抗体夹心酶联免疫吸附检测法;并与IBL公司PGRP试剂盒对比检测结果。结果新型ELISA检测标准抗原浓度范围为33~1.7×104pg/m L,检测小细胞肺癌患者血清PGRP浓度灵敏度100%,特异度98.4%,IBL试剂盒检测灵敏度100%,特异度92.2%。结论新型ELISA方法可用于小细胞肺癌患者血清PGRP浓度检测。Objective The value of pro-gastrin releasing peptide （ PGRP） which is the tumor marker of small cell lung canc-er has become a hot topic in recent years .The research was to build a new enzyme-linked immune sorbent assay （ ELISA） method ai-ming at detecting the concentration of PGRP in patients′serum. Methods We utilized synthetic PGRP epitopes for the screening of the monoclonal antibodies , labeled the screened monoclonal antibodies with horseradish peroxidase by modified sodium iodide method , and then established double antibody sandwich ELISA which could be used to detect the serum concentrations of PGRP in cancer pa -tients. Results We successfully screened E 12 mAb which could be served as the coating antibody and ED 1 mAb as the labeled anti-body.The standard antibody density range of new ELISA was 33 pg/mL^1.7 ×104 pg/mL.The comparison experiments between our method and the commercially available ELISA kit showed no significant difference （ P〉0.05）.The specificity of our method was 50%, and the sensitivity was 100%, while IBL kit was 92.2% and 100% respectively. Conclusion New ELISA can be used to detect the serum PGRP concentration in patients with small cell lung cancer .

关 键 词：胃泌素释放肽前体 辣根过氧化物酶 单克隆抗体 酶联免疫吸附试验 肿瘤 

分 类 号：R34[医药卫生—基础医学]