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作 者:黄金萍[1] 黄建邦[1] 高娃[1,2] 曾丽霞[1] 黄有明 宋银平[1] 岑伊静[1]
机构地区:[1]华南农业大学资源环境学院,广东广州510642 [2]包头市农业科学研究所,内蒙古包头014060
出 处:《华南农业大学学报》2015年第1期71-74,共4页Journal of South China Agricultural University
基 金:广东省教育厅2012年"广东省质量工程项目创新训练项目";公益性行业(农业)科研专项(201003067-01)
摘 要:【目的】明确柑橘木虱Diaphorina citri获毒的最佳取食部位和最短吸食时间,为生产实践中防治柑橘黄龙病提供依据.【方法】通过PCR测定了健康的柑橘木虱取食感染黄龙病的马水橘Citrus reticulata Blanco.cv.Mashuiju和酸橘Citrus sunki Hort.ex Tanaka的嫩梢、成熟叶和老叶24 h后的获菌效率.【结果和结论】取食马水橘3个部位的获菌效率差异显著:成熟叶>嫩梢>老叶.取食酸橘3个部位的获菌效率也有差异:老叶>成熟叶>嫩梢,其中老叶和成熟叶的获菌效率均显著高于嫩叶,但前两者差异不显著.对柑橘木虱取食2个品种的3个部位进行比较,取食马水橘嫩梢的获菌效率显著高于酸橘,而取食酸橘老叶的获菌效率则显著高于马水橘,取食2个品种成熟叶片的获菌效率差异不显著,2个品种整株总的获菌效率相同,均为36.67%.【Objective】To clarify the best feeding sites and the shortest time of Diaphorina citri Kuwayama in spreading Huanglongbing( HLB) and to provide guidance for the prevention and control of HLB.【Method】The bacterium acquisition efficiency of D. citri was tested by PCR,which had been fed on three different parts: tender shoots,mature leaves and old leaves of infected Citrus reticulata Blanco. cv.Mashuiju and Citrus sunki Hort. ex Tanaka for 24 h. 【Result and conclusion】The results showed that the infected rates of D. citri were significantly different among the three parts of C. reticulata. The infected rate on mature leaves was the highest and old leaves was the lowest. The bacterium acquisition efficiencies were also different among the three parts of C. sunki. The infected rates of old and mature leaves were both significantly higher than those of tender shoots. There was no significant difference between the old and mature leaves. The bacterium acquisition efficiency of D. citri feeding on tender shoots of C. reticulata was significantly higher than that on C. sunki,while on old leaves of C. sunki were significantly higher than that on C. reticulate,and there was no significant difference between the 2 species whenfeeding on mature leaves. The two hosts had the same infected rate of 36. 67% when compared feeding on the whole plants.
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