干旱胁迫对2种速生树种叶绿素荧光特性的影响  

Effects of drought stress on chlorophyll fluorescence parameters of two fast-growing tree species

作　　者：白晶晶[1] 吴俊文[1] 李吉跃[1] 何茜[1] 邱权[1] 潘昕[1] 

机构地区：[1]华南农业大学林学院,广东广州510642

出　　处：《华南农业大学学报》2015年第1期85-90,共6页Journal of South China Agricultural University

基　　金：高等学校博士学科点专项科研基金(20124404110007);亚热带农业生物资源保护与利用国家重点实验室开放课题(KSL-CUSAb-2012-07)

摘　　要：【目的】对华南地区2个速生树种尾巨桉Eucalyptus urophylla×E.grandis和竹柳(bamboo willow)的抗旱性进行比较.【方法】采用干旱胁迫处理,利用脉冲调制式叶绿素荧光仪(OS5P-美国)对2树种叶绿素荧光参数进行测定.【结果和结论】在干旱胁迫条件下,实际光量子产量(Yield)、表观光合电子传递速率(ETR)、光化学淬灭系数(q P)、最大荧光(Fm)和PSⅡ最大光化学效率(Fv/Fm)均有不同程度的下降,在干旱处理第24天,尾巨桉ETR比对照下降48.02%,而竹柳仅下降25.12%;到重度干旱时期,尾巨桉和竹柳Fv/Fm分别下降31.55%和21.84%;供试树种的非光化学淬灭系数(q N)、初始荧光(F0)均呈上升趋势,与对照相比,竹柳q N上升幅度为217.59%,显著大于尾巨桉(146.40%,P<0.05),F0上升幅度(49.11%)小于尾巨桉(92.03%);2种树种ETR与土壤含水量均呈极显著正相关(P<0.01),尾巨桉F0与土壤含水量呈极显著负相关(P<0.01),竹柳Fm和Fv/Fm与土壤含水量呈极显著正相关(P<0.01).研究结果表明:在干旱胁迫下,竹柳与尾巨桉叶绿素荧光参数间均表现一定相关性,相比尾巨桉,竹柳在叶绿素荧光参数上表现出更强的抗旱性.【Objective】A comparison of drought resistance was made between two fast-growing species in South China,Eucalyptus urophylla × E. grandis（ EE for abbr.） and bamboo willow（ BW for abbr.）.【Method】Effective quantum yield of PS Ⅱ photochemistry（ Yield）,apparent electron transport rate（ ETR）,the photochemical quenching（ q P）,the non-photochemical quenching（ q N）,minimal fluorescence（ F0）,maximal fluorescence（ Fm）,potential photochemical efficiency of PSⅡ（ Fv/ Fm） in EE and BW were tested by OS5 P pulse modulated chlorophyll fluorometer under drought stress. 【Result and conclusion】Yield,ETR,q P,Fmand Fv/ Fmall declined during the drought. At day 24 after treatment,ETR declined by 48. 02% and 25. 12% EE and BW,respectively. q N and F0 in the 2 species increased. q N in BW rose by 217. 59%,which was significantly higher than that in EE（ 146. 40%,P〈0. 05）. The relative increment in F0 in BW（ 49. 11%） was smaller than that in EE（ 92. 03%） at the end of the drought treatment. Correlation analyses were made concerning chlorophyll fluorescence charac-teristics and soil water content. In both species,ETR showed a significant positive correlation with soil water content（ P〈0. 01）. F0 in EE showed a significant negative correlation with soil water content（ P〈0. 01）. Fmand Fv/ Fmof BB and soil water content showed a significant positive correlation（ P〈0. 01）. These results indicate that correlations existe among chlorophyll fluorescence characteristics in two species under drought. BW has greater drought resistance than EE as revealed by chlorophyll fluorescence characteristics.

关 键 词：竹柳 尾巨桉 干旱胁迫 叶绿素荧光 

分 类 号：Q945.11[生物学—植物学]