猪博卡病毒的基因分型  

Studies on Genotyping of Porcine Bocaviruses

作　　者：周宇[1] 唐连飞[2] 朱事康[1] 朱中武[2] 佟铁铸[1] 禹思宇[2] 林志雄[3] 刘星[1] 陈燕忠[1] 罗卓军[1] 

机构地区：[1]惠州出入境检验检疫局技术中心,广东惠州516006 [2]湖南出入境检验检疫局,湖南长沙410004 [3]广东出入境检验检疫局,广东广州510623

出　　处：《中国动物检疫》2015年第2期63-68,共6页China Animal Health Inspection

基　　金：国家质检总局项目(2014IK243)

摘　　要：为了解猪博卡病毒(PBoV)的遗传进化规律,研究可行的PBoV基因分型方法,本研究利用Gen Bank中登录的29条PBoV和2条本实验室测得的全基因组序列信息,采用生物信息学软件进行基因组序列分析。结果显示,分别以NS1基因、NP1基因和VP1基因编码氨基酸构建的系统进化树,PBoV均被划分为3个基因群,但三种氨基酸序列绘制的进化树存在差异;基因群内各病毒株的氨基酸序列同源性较高,而各基因群之间病毒株的氨基酸序列同源性非常低,基因组结构差异较大。To analyze the genomic characteristics and classify the genotypes of porcine Bocavirus （PBoV） , the complete genomic sequences of 29 PBoVs available in GenBank and 2 PboVs detected by our laboratory were analyzed for the homology ofNS1, NP1, VP1 amino acid sequences by bioinformatics soRware. The results showed that PBoV could be divided into three groups based on phylogenetic tree established according to PBoV NS1, NP1, VP1 amino acid sequences, but there were also some noticeable differences among them： the viruses in the same group shared higher sequence identity and those in different groups shared lower sequence identity.

关 键 词：猪博卡病毒 遗传进化分析 基因分型 

分 类 号：S852.651[农业科学—基础兽医学]