生物膜富集系统的同步短程硝化反硝化研究  

Launch of Short-cut Nitrification-Denitrification in Biofilm Reactors under Low Dissolved Oxygen Condition

作　　者：何露露[1] 廖明军[1,2] 谢从新[1] 陆诗敏[1] 李大鹏[1] 

机构地区：[1]华中农业大学水产学院,湖北武汉430070 [2]湖北工业大学资源与环境工程学院,湖北武汉430068

出　　处：《环境科学与技术》2015年第1期80-84,99,共6页Environmental Science & Technology

基　　金：国家大宗淡水鱼类产业技术体系(nycytx-49-09);公益性行业(农业)专项(201203083);"十二五"农村领域国家科技计划课题(2012BAD25B01)

摘　　要：在自制生物膜反应器中接种精养鱼塘底泥进行富集培养,以期启动同步短程硝化反硝化过程。富集过程中记录反应器进、出水DO、NH4+-N、NO2--N和NO3--N变化。经过179 d运行后,反应器NH4+-N去除率达80%以上,出水NO2--N浓度非常低,低至检测极限以下,且没有NO3-积累。实时荧光定量PCR技术定量富集培养前后研究氨氧化细菌、反硝化细菌密度和厌氧氨氧化细菌数量,发现反应器中氨氧化细菌和反硝化细菌浓度分别增加了53.4倍和8.3倍,未检出厌氧氨氧化菌。综合上述结果判断生物膜反应器内启动了同步短程硝化反硝化过程。A biofilm reactor was developed to cultivate activated aquiculture pond sediments continuously under low DO condition of 0.4~1.1 mg/L. Concentration of ammonia,nitrite and nitrate in the reactor were monitored during the cultivation.After 179 days of cultivation,the removal rate of ammonia could reach above 80%,and the concentration of the effluent nitrite decreased to nearly 0 mg/L. During the cultivation,no nitrate accumulated in reactor. The contents of ammonia oxidizing bacteria（AOB）,denitrifying bacteria and anaerobic ammonia oxidizing bacteria（Anammox bacteria） in the reactor were determined through quantitative PCR. At the end of cultivation,the contents of AOB and denitrifying bacteria in reactor increased 53.4 and 8.3 times respectively,but Anammox bacteria was not detected. The results suggested that the short-cut nitrification-denitrification was activated in the reactor.

关 键 词：生物膜反应器 短程硝化反硝化 荧光定量PCR 

分 类 号：X703.1[环境科学与工程—环境工程]