血脂康对db/db糖尿病小鼠胰岛分泌功能和氧化应激影响的研究  

The effects of Xuezhikang on pancreatic islet function and oxidative stress in db/db diabetic mice

作　　者：王俊[1] 张珍[2,3] 邵加庆[4] 蒋卫民[5] 顾萍[4] 江时森[1] 

机构地区：[1]南京大学临床学院(南京军区南京总医院)心内科,210002 [2]南方医科大学南京临床医学院 [3]南京军区南京总医院内分泌科 [4]南京大学临床学院(南京军区南京总医院)内分泌科 [5]江苏省中医院心内科

出　　处：《中国糖尿病杂志》2015年第2期169-173,共5页Chinese Journal of Diabetes

基　　金：国家自然科学基金(81000352);江苏省自然科学基金(BK20140733)

摘　　要：目的探讨血脂康对db/db糖尿病小鼠胰岛分泌功能和氧化应激（OS）的影响。方法选取db/db小鼠40只,随机分为血脂康组和db/db组,各20只,另选取db/m小鼠20只作为对照（NC）组,分别给予血脂康和安慰剂灌胃。每周监测代谢指标,8周后行腹腔葡萄糖耐量试验（IPGTT）,离体胰岛灌流评价胰岛分泌功能,免疫组织化学法分析胰岛内8-羟-2c-脱氧鸟苷（8-OHdG）,4-羟壬烯醛（4-HNE）和gp91phox的表达水平。结果干预8周后,血脂康组FPG和FIns均低于db/db组[FPG：（18.4±3.4）vs（25.9±2.2）mmol/L;FIns：（7.7±0.8）vs（9.8±1.4）mIU/L,P〈0.05]。IPGTT糖负荷后各时间点,血脂康组葡萄糖曲线下面积（AUCg）均低于db/db组[（1800.7±187.1）vs（2550.0±179.6）,P〈0.05],而胰岛素曲线下面积（AUCi）显示,30min时AUCi 0-30高于db/db组[（285.3±8.9）vs（268.5±10.4）,P〈0.05]。与db/db组比较,血脂康组在给予16.7mm高糖刺激1min后胰岛素分泌升高。免疫组织化学法显示,与db/db组比较,血脂康组胰岛内8-OhdG,4-HNE和gp91phox的表达降低更明显（P〈0.05）。结论血脂康能降低胰岛OS反应,并改善葡萄糖代谢。Objective To investigate the effects of Xuezhikang on pancreatic islet endocrine function and local oxidative stress in db/db diabetic mice. Methods A total of 40 male genetically diabetic C57BL/KsJ-db/db mice were randomly assigned into xuezhikang group treated with xuezhikang at 300mg/（kg·d）and db/db group treated with placebo.In addition,another 20 age-matched lean non-diabetic C57BL/KsJ-db/m littermates served as normal control（NC）group.The metabolic indexes were measured every week.After 8 week,10 mice were randomly selected from each group and received intraperitoneal glucose tolerance test（IPGTT）.The in vitro insulin release kinetics was evaluated by perifusion system and the expression levels of gp91 phox,8-OhdG and 4-HNE were analyzed by immunohistochemical method. Results After 8 week treatment,FPG and FIns were lower in Xuezhikang group than in db/db group[FPG：（18.4±3.4）vs（25.9±2.2）mmol/L;FIns：（7.7±0.8）vs（9.8±1.4）mIU/L,P〈0.05].At the various time points after IPGTT,the AUCgwas lower in Xuezhikang group than in db/db group[（1800.7±187.1）vs（2550.4±179.6）,P〈0.05],and at the 30 minute after IPGTT,the AUCiwas higher in Xuezhikang group than in db/db group[（285.3±8.9）vs（268.5±10.4）,P〈0.05].Compared with the db/db group,since 1 minute point after high-concentration glucose stimulation,the insulin level increased remarkably in Xuezhikang group.Immunohistochemistry method showed that compared with db/db group,xuezhikang group showed the expression of gp91 phox,8-OhdG and 4-HNE was reduced（P〈0.05）. Conclusion Xuezhikang can inhibit the local oxidative stress of pancreatic islet and improve glucose metabolism in db/db diabetic mice.

关 键 词：糖尿病 血脂康 胰岛分泌功能 氧化应激 

分 类 号：R285.5[医药卫生—中药学]