冷藏对血小板膜糖蛋白GPⅠbα末端糖基脱落的影响  

Effect of refrigerated storage on platelet membrane glycoprotein GPⅠbα terminal sugar radicals

作　　者：杨玲玲[1] 石丽萍[1] 龚晓燕[1] 陈立[1] 任芙蓉[1] 

机构地区：[1]北京市红十字血液中心安全输血室,100088

出　　处：《北京医学》2015年第2期157-159,共3页Beijing Medical Journal

基　　金：首都卫生发展科研专项(首发2011-1019-01)

摘　　要：目的测定冷藏(4℃)保存48 h后血小板膜糖蛋白GPⅠbα末端糖基脱落情况。方法分别以一定浓度梯度的FITC-s WGA、FITC-RCA-1、FITC-ECA标记血小板,应用流式细胞仪检测,确定最佳工作浓度。检测冷藏保存48 h后血小板GPⅠbα末端唾液酸基及次末端半乳糖基脱落情况。结果 FITC-s WGA、FITC-RCA-1、FITC-ECA工作浓度分别为2μg/ml、2μg/ml、30μg/ml时标记效果好。冷藏保存48 h后GPⅠbα末端唾液酸基及次末端半乳糖基脱落较22℃常规保存明显增加,分别增至(156±18)%及(123±13)%,差异有统计学意义(P<0.01)。结论冷藏保存48 h可致血小板膜糖蛋白GPⅠbα末端糖基脱落增加。Objective To measure the exposed residues of platelet membrane glycoprotein GPⅠbαwith flow cy-tometry （FCM） after refrigerating 48 h. Methods A series of concentration was set, and the optimal working concentration of FITC-sWGA, FITC-RCA-1, FITC-ECA was determined respectively. The platelet membrane GPⅠbαterminal exposed residues were analyzed after 22℃ preservation and 4℃ refrigeration respectively. Results FITC-sWGA, FITC-RCA-1 and FITC-ECA worked well at 2μg/ml, 2μg/ml and 30μg/ml respectively. Refrigeration led to increasing of GPⅠbαterminal sugar radicals shedding, terminal sialic acid group shedding increased to （156±18）%（P〈0.01）, and penultimate galactose group shedding increased to （123 ±13）%（P〈0.01）. Conclusion Refrigerating platelets for 48 h leads to in-creasing of platelet GPⅠbαsubunit terminal sugar radicals shedding.

关 键 词：血小板保存 血小板膜糖蛋白 流式细胞术 

分 类 号：R457.1[医药卫生—治疗学]