碳离子辐照对猪链球菌部分生物学特性的影响  

The influence of some biological characteristics on streptococcus suis irradiated by carbon ion

作　　者：陆萍[1] 焦安心 常沛玺 胡步荣[2] 付谦[1] 孙裴[1] 魏建忠[1] 李郁[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]中国科学院近代物理研究所,兰州730000

出　　处：《辐射研究与辐射工艺学报》2015年第1期51-57,共7页Journal of Radiation Research and Radiation Processing

基　　金：安徽省长三角联合攻关项目(1101c0603065);安徽农业大学学科学位点建设项目(XKXWD2013006);安徽农业大学大学生创新基金项目(2011053);安徽省生猪产业体系基金联合资助

摘　　要：为了解30 Gy和50 Gy 12C6+离子辐照对猪链球菌部分生物学特性的影响,采用微生物学法和毒理学法检测亲本株和突变株的溶血性、对18种抗生素的敏感性、生物被膜形成能力、表面疏水性及毒力。结果显示,与亲本株相比,30 Gy突变株的溶血性、对抗生素的敏感性、生物被膜形成能力,表面疏水性及毒力无显著差异(p≥0.05);50 Gy突变株对庆大霉素、复方新诺明、链霉素、甲氧苄啶和洁霉素的敏感性差异显著(p≤0.05),但溶血性、生物被膜形成能力、表面疏水性及毒力无显著差异(p≥0.05)。结果表明,30 Gy 12C6+离子辐照不能导致猪链球菌生物学特性显著改变,而50 Gy 12C6+离子辐照猪链球菌能够导致部分突变株对部分抗生素敏感性的显著改变,但不同突变株对不同种类抗生素的敏感性改变不同。In order to investigate the biological characteristics changes of Streptococcus suis after being irradiated by 30 Gy, 50 Gy ^12C^6＋ ion, Microbiological methods and Toxicology methods were used to detect hemolytic, antibiotic sensitivity of 18 kinds of antibiotics, biofilm formation ability, surface hydrophobic, and virulence of parent Streptococcus suis strain and mutant strain. Compared with the parent strains, the results showed that there was no significant difference in 30 Gy ^12 C^6＋ ion group in the aspect of hemolysis, antibiotic sensitivity, ability of biofilm formation, surface hydrophobicity and virulence （p≥0.05）; the antibiotic sensitivity of Streptococcus to Trimesulf, Streptomycin, Trimethoprim, Gentamycin, Lincomycin in 50 Gy ^12C^6＋ ion group was of significant difference （p≤0.05）, but there was no significant difference in hemolytic, biological film forming ability, surface hydrophobicity and virulence （p≥0.05）. In conclusion, there was no significant change in biological characteristics of Streptococcus suis after irradiation with 30 Gy ^12C^6＋ ion, significant changes of the some antibiotic sensitivity were found in 50 Gy ^12C^6＋ ion irradiation group, but different mutations change differently in sensitivity to different antibiotics.

关 键 词：碳离子 辐照 猪链球菌 生物学特性 影响 

分 类 号：TL72[核科学技术—辐射防护及环境保护]