海洋来源真菌Aspergillus sp.SCSIO 81-F2抗菌活性次级代谢产物的研究  

Antibacterial Secondary Metabolites from Marine-derived Fungus Aspergillus sp. SCSIO 81-F2

作　　者：罗明和[1,2] 黄洪波[2] 李沉纹[1] 于彩平[1] 马俊英[2] 卢来春[1] 鞠建华[2] 

机构地区：[1]第三军医大学大坪医院野战外科研究所药剂科,重庆400042 [2]中国科学院热带海洋生物资源与生态重点实验室中国科学院海洋微生物中心广东省海洋药物重点实验室中国科学院南海海洋研究所,广州510301

出　　处：《天然产物研究与开发》2015年第2期241-245,共5页Natural Product Research and Development

基　　金：国家国际科技合作专项(2011DFA32540);国家863项目(2012AA092104);重庆市卫生局科技项目(ZY20132062)

摘　　要：采用抑菌活性追踪的方法,利用硅胶柱色谱和半制备反相高效液相色谱法对一株南海海洋来源真菌SCSIO 81-F2的发酵产物进行分离,纯化获得3个单体化合物,通过理化性质、MS、NMR及X-单晶衍射分析,分别鉴定为xanthocillin X dimethylether(1)、xanthocillin X monomethylether(2)、6',8'-dimethoxy xanthocillin X dimethylether(3)。经18S r DNA序列分析并构建系统发育进化树,鉴定该菌株为曲霉属真菌Aspergillus sp.SCSIO 81-F2。海洋曲霉菌Aspergillus sp.SCSIO 81-F2是一个能产生具有抗菌活性xanthocillin类抗生素的新来源。Guided by antibacterial assay,the chemical components of the fermentation products of strain SCSIO 81-F2, which was isolated from a sediment collected in the South China Sea, were isolated and purified using silica gel column chromatography and semi-preparative HPLC. Three known compounds, xanthoeiilin X dimethylether （1）, xanthocillin X monomethylether （2） ,6＇ ,8＇-dimethoxy xanthocillin X dimethylether （3） were obtained from the fermentation products. The structures of the purified compounds were identified based on MS and NMR spectroscopic analysis and X-ray crys- tallography. This fungus was identified as Aspergillus sp. SCSIO 81-F2 on the basis of phylogenetie tree analysis of its 18S rDNA sequence, which was a new source of producing xanthociUin X type compounds with antibacterial acitivity.

关 键 词：Xanthocillin X 海洋真菌 曲霉菌属 次级代谢产物 

分 类 号：O629[理学—有机化学]