响应面法优化泡桐叶中熊果酸的超声波提取工艺  

Optimization of Ultrasonic Extraction of Ursolic Acid from Paulownia elongata Leaves by Response Surface Methodology

作　　者：邢雅丽[1,2] 毕良武[1,2,3] 赵振东[1,2,3] 夏田娟 

机构地区：[1]中国林业科学研究院林产化学工业研究所生物质化学利用国家工程实验室国家林业局林产化学工程重点开放性实验室 [2]江苏省生物质能源与材料重点实验室,南京210042 [3]中国林业科学研究院林业新技术研究所,北京100091

出　　处：《天然产物研究与开发》2015年第2期301-305,共5页Natural Product Research and Development

基　　金：引进国际先进林业科学技术项目(2011-4-01)

摘　　要：为探讨泡桐叶中熊果酸的超声波提取工艺,通过中心复合设计-响应面法研究乙醇浓度(X1)、液固比(X2)、超声波提取时间(X3)对泡桐叶中熊果酸提取得率的影响。结果表明,各因素对熊果酸得率的影响顺序为乙醇浓度(X1)>超声波提取时间(X3)>液固比(X2);超声波辅助提取泡桐叶中熊果酸的最优工艺条件为:乙醇浓度89%,液固比31 m L/g,超声时间37 min;在此条件下,熊果酸得率为14.80 mg/g,与预测值相近,二次回归模型预测性良好。In order to investigate ultrasonic extraction technology of ursolic acid from Paulownia elongate leaves,central composite design coupled with response surface analysis was adopted to study the effects of ethanol concentration （ Xt ）, ratio of liquid to solid （ Xz ） and ultrasonic time （ X3 ） on the extraction yield of ursolic acid. The results showed that the effects of ultrasonic factors on the extraction yield of v.molic acid from P. elongate leaves were in the following order：eth- anol concentration （XI） 〉 ultrasonic time （X3 ） 〉 ratio of liquid to solid （Xa ） ,and the optimal extraction conditions were as follows：89% ethanol solution as extraction solution,30 mL/g as the ratio of liquid to solid,37 rain as ultrasonic time. Under the optimal conditions,the extraction yield of umolic acid reached 14.80 mg/g,which was close to the pre- dicted response value. Hence, the quadratic regression model can be used to predict the ultrasonic extraction conditions of ursolic acid from P. elongate leaves.

关 键 词：中心复合设计 泡桐叶 超声波提取 熊果酸 

分 类 号：R284.2[医药卫生—中药学]