响应面优化超声辅助提取刺梨多糖工艺研究  

Optimization of Ultrasonic-assisted Extraction of Polysaccharides from Rosa roxburghii Tratt Using Response Surface Methodology

作　　者：唐健波[1,2] 肖雄[1] 杨娟[1] 彭梅[1] 刘建华[1] 

机构地区：[1]贵州省中国科学院天然产物化学重点实验室,贵阳550002 [2]贵州省生物技术研究所,贵阳550006

出　　处：《天然产物研究与开发》2015年第2期314-320,共7页Natural Product Research and Development

基　　金：973计划前期研究专项(2012CB722601)

摘　　要：探讨超声波作用下刺梨多糖提取的最佳工艺条件。在单因素试验基础上,采用响应面法对刺梨多糖提取工艺参数进行优化研究。响应面试验表明提取温度、超声功率、超声时间、液料比对响应值刺梨多糖提取率均有显著影响,优化得到超声辅助提取刺梨多糖最佳工艺条件为:超声时间30 min,超声功率120 W,液料比40m L/g,提取温度80℃,提取3次。在此条件下的刺梨多糖提取率可达2.18%,与模型预测值非常接近。The aim of this study was to obtain the optimal condition of ultrasonic-assisted extracting polysaccharides from Rosa roxburghii Tratt. On the basis of single factor test, the response surface methodology was applied to optimize the main extraction conditions. The results showed that the optimized conditions were：ultrasonic time 30 min, ultrasonic pow- er of 120 W,liqnid to solid ratio of 40 mL/g,extraction temperature of 80 ℃ and with 3 times extraction. Under these optimized conditions,the extraction yield of polysaccharide was 2.18% ,exhibiting a good agreement with the predicted value.

关 键 词：刺梨多糖 超声提取 响应面法 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]