冻干血小板对SD大鼠烫伤创面的修复作用研究  

Repair effect of freezee-dried platelets on scalds in SD rats

作　　者：李文丹[1,2] 单桂秋[2] 马静[2] 林放[2] 张婷婷[2] 吕品[2] 周谋[2] 黎诚耀[1] 

机构地区：[1]南方医科大学生物技术学院输血医学系,广东广州510515 [2]广州军区广州总医院输血科

出　　处：《中国输血杂志》2015年第1期11-14,1,共4页Chinese Journal of Blood Transfusion

基　　金：国家科技重大专项课题(2011ZXJ09104-07C)

摘　　要：目的探讨冻干血小板对SD大鼠深Ⅱ度烫伤模型创面愈合的影响。方法将40只SD大鼠(雄性)随机分成4组(n=10):冻干血小板(FDP)组、新鲜血小板(FPRP)组、烫伤膏组、生理盐水组。采用煮沸砝码加压法建立大鼠深Ⅱ度烫伤模型,分组给药,1次/d,连续20 d。记录动物烫伤模型创面愈合情况并做组织学检查。结果给药至d7,4组大鼠烫伤创面愈合率分别为:FDP组(41.27±5.90)%、FPRP组(41.62±13.93)%、盐水组(33.52±14.58)%、烫伤膏组(33.58±10.89)%,FDP组和FPRP组创面愈合率明显高于烫伤膏组和生理盐水组(P<0.05);病理组织学检查:给药d7、d21,冻干组和新鲜组对大鼠烫伤创面皮肤病理的修复作用明显优于烫伤膏组及盐水组,而这2组血小板组间比较无明显差异。结论冻干血小板促进大鼠烫伤模型创面早期愈合作用与新鲜血小板相近。Objective To observe the efficacy of freeze-dried platelets （FDP） on the deep second-degree scalded model in SD rats. Methods 40 SD rats, male , were marked and divided randomly into 4 groups ： the FDP group, the fresh plate- let-rich plasma（FPRP） group, scalds ointment（SO） group and blank control group. After heated in boiling water, weight was put on the skin of mice＇ s back to establish the model of superficial Ⅱ -degree scald. Drug delivery to all rats was once a day for 20 consecutive days. Recorded information on the wound healing and made histological examination. Results At day7,the wound healing rate of forth group respectively was： FDP group （41.27 ± 5.90）%, FPRP group （41.62 ± 13.93） %, blank control group （ 33.52 ± 14. 58 ） %, SO group （ 33.58 ± 10. 89 ） %, the wound healing in FDP group and FPRP group was significantly higher than SO group and blank control group （ P 〈 0.05 ）. Meanwhile, histopathology results also suggest that, at day 7 and day 21, the scald wound repairing effect in FDP and FPRP groups is superior to SO group and blank control group. However, there were no differences between FDP group and FPRP group. Conclusion Similar with FPRP, FDP could also accelerate the healing of scald wound.

关 键 词：冻干血小板 新鲜血小板 烫伤 创面愈合 动物模型 SD大鼠 

分 类 号：R331.143[医药卫生—人体生理学] R457.1[医药卫生—基础医学]