高效液相色谱法检测饲料中三唑类杀菌剂残留  

Simultaneous determination of four triazole fungicide residues in feeds by high performance liquid chromatography

作　　者：赵春娟[1,2] 高文惠[1,2] 

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018 [2]河北省发酵工程技术研究中心,河北石家庄050018

出　　处：《中国饲料》2015年第3期24-25,34,共3页China Feed

基　　金：河北省科技支撑计划项目(14227504D;14236602D-13)

摘　　要：本实验建立了同时分离检测饲料样品中腈菌唑、戊唑醇、联苯三唑醇、烯唑醇4种三唑类杀菌剂残留的高效液相色谱法。使用C18色谱柱,以甲醇-水（85∶15,V/V）溶液为流动相,紫外检测波长为210 nm,外标法定量。结果表明,该方法在9 min内实现了4种三唑类杀菌剂的基线分离,4种杀菌剂的平均回收率为83.4%～101.2%,相对标准偏差（RSDs）〈3.00%（n=5）。A high performance liquid ehromatography（HPLC）method was developed to detect myclobutanil,tebucona- zole,bitertanol and diniconazole residues in feeds.The samples were separated using C18 column with a mobile phase of methanol and water （85：15, V/V）and the detection wavelength of 210 nm, and were quantified by external standard method. The results showed that the four triazole fungicides were separated on baseline within 9 rain ,the average recoveries of the four fungicides were between 83.4% and 101.2%, and the relatives standard deviations（RSDs）were less than 3.00% （n=5）.

关 键 词：饲料 三唑类杀菌剂 残留 高效液相色谱法 

分 类 号：S816.17[农业科学—饲料科学]