薏苡仁酯对胶原诱导性关节炎小鼠Th17细胞分化作用研究  

Effect of Coixenolide on Differentiation of T Helper Cell 17 in Mice with Arthritis Induced by Collagen

作　　者：郑红霞[1] 章伟明[2] 周红娟[1] 张雯[1] 于健宁[1] 汪卫[1] 

机构地区：[1]浙江省中西医结合医院风湿免疫科,杭州310003 [2]杭州市第一人民医院中药房,杭州310003

出　　处：《浙江中西医结合杂志》2015年第2期118-119,123,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药科技计划项目(No.2011ZB106)

摘　　要：目的研究薏苡仁酯对胶原诱导性关节炎（CIA）小鼠体内Th17细胞及其细胞因子白细胞介素-17（IL-17A）的作用。方法健康SPF级DBA/1 7~8周雄性小鼠20只,测定造模对照组（经生理盐水治疗后CIA小鼠）、薏苡仁酯组（经薏苡仁酯注射液治疗后CIA小鼠）小鼠脾脏Th17细胞及血清中IL-17变化。应用流式细胞术（FCM）分析小鼠脾脏中Th17细胞的比例,采用酶联免疫吸附试验（ELISA）检测小鼠血清中IL-17A含量。结果经薏苡仁酯注射液治疗后,薏苡仁酯组CIA小鼠脾脏中Th17细胞比例较造模对照组显著降低（1.82%比4.99%,P〈0.05）,小鼠血清中IL-17A的含量也明显低于造模对照组[（3.54±0.61）pg/m L比（8.31±0.25）pg/m L,P〈0.05]。结论薏苡仁酯对CIA小鼠Th17细胞具有分化作用。Objective To explore the effect of coixenolide on T helper cell 17（Th17） and its cytokine interleukin-17（IL-17A） in mice with collagen-induced arthritis（CIA）. Methods Twenty DBA/1 mice at age of 7-8weeks were used to build CIA model, then they were randomly divided into model group and coixenolide-treated group, with 10 mice in each group. Mice in coixenolide-treated group was injected with 25 ml/kg coixenolide and mice in model group were given injection of equivalent normal saline. The proportion of Th17 cells in the spleen of mice and serum IL-17 A were determined by flow cytometry and enzyme-linked immunosorbent assay（ELISA）,respectively. Results The proportion of Th17 in the spleen of mice in coixenolide-treated group was significantly lower than that in model group（1.82% vs 4.99%, P 〈0.05）. The serum IL-17 A of mice in coixenolide-treated group was significantly lower than that in control group [（3.54±0.61）pg/m L vs（8.31±0.25）pg/m L,P〈0.05]. Conclusion Coixenolide has inhibitory effect on differentiation of Thl7 cells in CIA mice.

关 键 词：小鼠 胶原诱导性关节炎 薏苡仁酯 TH17细胞 白细胞介素-17 

分 类 号：R285.5[医药卫生—中药学]