不同环境下水稻株高和穗长的QTL分析  

Dissection of QTLs for Plant Height and Panicle Length Traits in Rice Under Different Environment

作　　者：姚晓云[1] 李清[1] 刘进[1] 姜树坤[2] 杨生龙[1] 王嘉宇[1] 徐正进[1] 

机构地区：[1]沈阳农业大学水稻研究所/农业部东北水稻生物学与遗传育种重点实验室,沈阳110866 [2]黑龙江省农业科学院耕作栽培研究所,哈尔滨150086

出　　处：《中国农业科学》2015年第3期407-414,共8页Scientia Agricultura Sinica

基　　金：辽宁省高等学校优秀人才支持计划(LJQ2013075)

摘　　要：【目的】水稻株高和穗长是影响水稻产量的2个重要因素,选育长穗大粒和株高适中的品种将对水稻的增产有非常重要的意义。通过对株高和穗长进行多环境QTL分析,鉴定稳定表达的株高和穗长的主效QTL,增加对株高和穗长遗传行为的了解,为水稻株型育种提供参考。【方法】首先,以辽宁省超级粳稻品种沈农265和云南省的地方粳稻品种丽江新团黑谷杂交衍生的粳-粳交重组自交系(recombinant inbredlines,RILs)群体为试验材料,采用QTL Ici Mapping v3.0软件基于完备复合区间作图法在多环境条件下(沈阳,2011;海南,2012年;沈阳,2013年)对株高和穗长进行QTL分析;其次,基于上面定位的结果,结合已发表的文献和水稻数据库中的相关数据,对在3种环境条件下检测到的主效QTL进行比较分析,确定其可靠性;最后,采用主效QTL-BSA法(Bulked Segregant Analysis of Major QTL)对3种环境条件下检测到的主效QTL进行分析,进一步缩小目标QTL的区间范围。【结果】在3种环境条件下,沈农265和丽江新团黑谷的株高和穗长均存在显著差异,在RILs群体中,株高和穗长存在较大幅度变异,呈现双向超亲分离,近似于正态分布,这表明株高和穗长均为多基因控制的数量性状。在3种环境下,共检测到9个与株高和穗长相关的QTL,包括5个株高QTL,分布于第6、7、9和12染色体上,LOD介于2.67—19.39,加性效应值在-17.68—2.90,单个QTL贡献率为4.25%—37.35%;4个穗长QTL,分布于第6、7和9染色体上,LOD介于3.57—23.18,加性效应值在-3.22—1.42,单个QTL贡献率为11.30%—61.62%。有5个QTL被单独检测到,仅有4个QTL能在2个或3个环境中被检测到。其中,位于第9染色体上相同区间的q PL9a和q PH9能在3种环境中被检测到,而位于第7染色体上相同区间的q PH7和q PL7b分别能在2种或3种环境中被检测到,增效等位基因均来自丽江新团黑谷。同时,依据已发表的相关文献和Gramene�【Objective】Panicle length and plant height are two important factors affecting rice production. Breeding varieties with large panicle and the ideal plant architecture traits are significant for increasing the grain yield of rice. To explore the genetic basis of plant height and panicle length in rice, quantitative genetic analysis was conducted in three different environments. In this study, stable expressed QTLs for plant height and panicle length were identified. The results of this study help us to understand the genetic basis of plant height and panicle length, and provide us with useful information for marker-assisted improvement of the plant type in rice.【Method】The recombinant inbred lines（RILs） derived from the cross between Shennong 265（japonica） and Lijiangxintuanheigu（japonica） consisting of 126 lines were used to identify QTLs for plant height and panicle length in three different environments（Shenyang, 2011; Hainan, 2012; Shenyang, 2013）. Nine QTLs were mapped by QTL Ici Mapping v3.0 software, using ICIM method for plant height and panicle length. In addition, based on the QTL results and the published literature and rice database data, the major QTLs in 3 environments were analyzed. These results were further confirmed the reliability of the QTL. Finally, in order to narrow the interval of the major QTLs, the QTL-BSA（Bulked Segregant Analysis of Major QTL） was used for fine mapping.【Result】Analysis of variance test showed that a significant difference was detected between the parents under three different conditions. A normal distribution of phenotypic values for panicle length and plant height were observed, which indicated that panicle length and plant height were controlled by multiple genes. A total of nine QTLs for the plant height and panicle length were mapped. Five QTLs affecting plant height were detected on the chromosomes 6, 7, 9 and 12, with the LOD values ranged from 2.67 to 19.39, the additive effect from-17.68 to 2.90, and the range of individually expl

关 键 词：水稻 株高 穗长 数量性状基因座 

分 类 号：S511.22[农业科学—作物学]