盐、碱胁迫条件下粳稻Na^+、K^+浓度的QTL分析  

QTL Analysis of Na^+ and K^+ Concentrations in Japonica Rice Under Salt and Alkaline Stress

作　　者：邢军[1] 常汇琳 王敬国[1] 刘化龙[1] 孙健[1] 郑洪亮[1] 赵宏伟[1] 邹德堂[1] 

机构地区：[1]东北农业大学农学院水稻研究所,哈尔滨150030

出　　处：《中国农业科学》2015年第3期604-612,共9页Scientia Agricultura Sinica

基　　金：国家"十二五"科技支撑计划(2011BAD16B11;2011BAD35B02-01-01);国家"十二五"科技支撑计划农村领域课题(2013BAD20B04)

摘　　要：【目的】水稻栽培区土壤的盐、碱化日趋严重,植物体内Na+、K+浓度及Na+/K+是植物耐盐、碱性重要指标。在盐、碱胁迫条件下检测水稻苗期地上部和根部的Na+、K+浓度及Na+/K+的QTL位点,为水稻的耐盐、碱性遗传机制及分子标记辅助育种提供理论依据。【方法】以优质高产水稻品种东农425与耐盐、碱水稻品种长白10为亲本构建重组自交系(RIL)为作图群体,利用102对SSR标记构建遗传连锁图谱,该图谱覆盖水稻基因组约1 915.05 c M,标记间平均距离为18.77 c M;在140 mmol·L-1 Na Cl盐胁迫和0.15%Na2CO3碱胁迫处理条件下,对水稻苗期地上部和根部的Na+、K+浓度及Na+/K+等性状进行测定,利用SPSS v19.0对各性状进行相关分析,并采用QTL Ici Mapping v3.3的完备区间作图法(ICIM)进行QTL定位。【结果】盐、碱胁迫条件下,亲本及RIL群体地上部Na+、K+浓度均高于地下部Na+、K+浓度,各性状在RIL群体中基本符合正态分布,表现出典型的数量性状遗传特征,符合QTL定位要求。相关分析结果表明,盐、碱胁迫条件下,地上部Na+与K+及根部Na+与K+均呈极显著正相关,2种胁迫条件下的各性状相关性不显著。盐、碱胁迫条件下共检测到15个与Na+、K+浓度和Na+/K+相关的QTL,2种条件下所检测到的QTL位于不同染色体区域。在盐胁迫下共检测到5个QTL,包括1个与地上部K+浓度相关QTL,位于第8染色体的RM1308—RM281区间内,贡献率为6.83%;3个与根部Na+浓度相关QTL,位于第3和第8染色体上,其中q SRNC3-1贡献率最大,为16.41%;1个与根部K+浓度相关QTL,贡献率为3.52%;未检测到与地上部Na+浓度、Na+/K+及根部Na+/K+相关的QTL。在碱胁迫下共检测到10个QTL,包括1个与地上部Na+浓度相关的QTL,位于第2染色体的RM1347—RM48区间内,贡献率为14.41%;1个与地上部K+浓度相关QTL,位于第2染色体的RM1255—RM213区间内;3个与地上部Na+/K+相关QTL,分别位于第2、7、10染色体上,其【Objective】Salinization and alkalinization of soil are becoming worse and worse during these years, and the concentrations of Na＋, K＋ and Na＋/K＋ ratio could be taken as the criterial indicators of saline-alkali tolerance in plant. Therefore, the QTL analysis of Na＋, K＋ concentration and Na＋/K＋ ratio in shoots and roots of rice at the seedling stage under salt or alkaline stress were conducted in the present study in order to provide a scientific basis for the rice genetic mechanism of salt and alkaline tolerance and molecular marker assisted breeding. 【Method】 The recombinant inbred line（RIL） population derived from a cross Dongnong 425（high yield and quality） as the female parent and Changbai 10（salt tolerance） as the male parent. A genetic linkage map was constructed with 102 SSR markers, covering 1 915.05 c M of rice genome at an average interval of 18.77 c M. The concentrations of Na＋, K＋ and Na＋/K＋ ratio in shoots and roots were phenotyped under 140 mmol·L-1 Na Cl of salt stress and 0.15% Na2CO3 of alkali stress at seedling stage. The correlation analysis by using SPSS v19.0 program and QTL analysis by using QTL Ici Mapping v3.3 program of the complete interval mapping method were conducted.【Result】The Na＋ and K＋ concentrations in shoots were higher than in roots under salt and alkaline stress in both the parents and RILs. All traits almost presented gaussian distribution, conforming typical genetic model of quantitative traits and the requirements of the QTL mapping. The results of correlation analysis showed that the concentrations of Na＋ and K＋ had a significant positive correlation in shoots and roots under salt and alkali stress, whereas there was no significant correlation between salt and alkali stress. A total of fifteen QTLs were detected under salt and alkaline stress, QTLs are located on different chromosomes between salt and alkali stress. Five QTLs were detected under salt stress, including one QTL associated with the concentration of K＋

关 键 词：粳稻 盐胁迫 碱胁迫 钠钾离子 SSR标记 QTL 

分 类 号：S511.22[农业科学—作物学]