铜绿丽金龟对植物源挥发物的触角电位和行为反应  

Electroantennographic and behavioral responses of Anomala corpulenta to plant volatiles

作　　者：谢明惠[1,2] 陈浩梁[2] 衣建坤[1] 李克斌[1] 张杰[1] 苏卫华[2] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]安徽省农业科学院植物保护与农产品质量安全研究所,合肥230031

出　　处：《植物保护》2015年第1期33-38,共6页Plant Protection

基　　金：公益性行业(农业)科研专项(201003025);植物病虫害生物学国家重点实验室开放基金课题(SKL2012IP07);安徽省农业科学院科技创新团队(11C1106);安徽省农业科学院院长青年创新基金面上项目(14B1150)

摘　　要：为筛选适宜的铜绿丽金龟植物源引诱剂,利用触角电位(EAG)和Y形嗅觉仪技术,测定了铜绿丽金龟雌、雄虫对20种植物源挥发物的电生理和行为反应。结果表明:在10μg/μL浓度下,铜绿丽金龟对甲基庚烯酮、顺-3-己烯乙酸酯、壬醛、1-辛烯-3-醇、芳樟醇和反-2-己烯醇具有较强的触角电位反应,且呈雌虫大于雄虫的趋势。Y形嗅觉仪行为测定中,雌虫对多数挥发物的敏感度高于雄虫。其中反-2-己烯醇、顺-3-己烯乙酸酯、甲基庚烯酮和1-辛烯-3-醇对铜绿丽金龟雌虫的吸引作用显著高于对照;而雄虫仅对顺-3-己烯乙酸酯有显著的趋向选择。本研究为开发有效的铜绿丽金龟田间引诱剂用于生态治理提供依据。To find possible attractants, 20 kinds of plant volatiles were chosen for electroantennogram test and Ytube olfactometer bioassays using both female and male Anomala corpulenta. The results showed that methyl heptenone, cis-3-hexenyl acetate, nonanal, 1-octene-3-alcohol, linalool and trans-2-hexenol elicited significant EAG responses at the concentration of 10 μg/μL; furthermore, the response of females was stronger than that of males. The Y-tube olfactometer bioassays indicated that females were more sensitive to most volatiles than males. Compared with the control, females were attracted by trans-2-hexenol, cis-3-hexenyl acetate, 6-methyl 5-hepten- 2-one and 1-octene-3-alcohol, but males were only attracted by cis-3-hexenyl acetate. This study could provide a theoretical basis for developing attractants of A. corpulenta for ecological management.

关 键 词：铜绿丽金龟 植物源挥发物 Y型嗅觉仪 触角电位技术 行为反应 

分 类 号：Q965[生物学—昆虫学]