茶刺蛾核型多角体病毒多角体的增殖动态与生产工艺  

The propagation dynamics and production process of the nucleopolyhedroviruses of the tea slug moth Iragoides fasciata

作　　者：唐美君[1] 殷坤山[1] 郭华伟[1] 肖强[1] 

机构地区：[1]中国农业科学院茶叶研究所,杭州310008

出　　处：《植物保护》2015年第1期174-177,共4页Plant Protection

基　　金：国家科技基础性工作专项(2013FY113200);浙江省茶产业科技创新团队项目(2011R50024)

摘　　要：为了解茶刺蛾核型多角体病毒（Iragoides asciata nucleopolyhedrovirus IrfaNPV）的增殖特性、指导茶刺蛾病毒的生产,采用室内活体增殖法,测定了IrfaNPV多角体在茶刺蛾幼虫体内的增殖动态,并设计了工艺流程,进行了大量繁殖生产试验.结果显示,IrfaNPV多角体在虫体内的增殖动态符合Logistic曲线,饲毒后9～12 d为病毒快速增殖期.按设计的生产工艺进行病毒大量繁殖生产,虫尸中病毒多角体平均可达8.3×10^8 PIB/头,茧中病毒多角体含量平均为6.4×10^8 PIB/头.该工艺可用于今后茶刺蛾病毒的大量生产.To guide the production of lragoides fascia ta nucleopolyhedrovirus（IrfaNPV）, the propagation dynamic of IrfaNPV was investigated in laboratory. Meanwhile, the production process was developed and the mass production of the virus was conducted according to the process. The results showed the propagation dynamic of IrfaNPV in the larval body accorded with logistic curve. The average yield of IrfaNPV reached 8.3× 10^8 PIB/larva and 6.4× 10^8 PIB/cocoon, respectively. The process is expected to be applied in mass production of IrfaNPV in the future.

关 键 词：茶刺蛾 核型多角体病毒 增殖动态 生产工艺 

分 类 号：S432.41[农业科学—植物病理学]