高效液相色谱法测定洗涤用品中的三氯生  

Determination of Triclosan in Detergents by High Performance Liquid Chromatography

作　　者：王晓娟[1,2] 林振兴[1,2] 黄通人[1,2] 

机构地区：[1]宁波出入境检验检疫局,浙江宁波315012 [2]浙江省石油化工产品质量与安全检测技术研究重点实验室,浙江宁波315012

出　　处：《化学试剂》2015年第2期139-141,154,共4页Chemical Reagents

基　　金：国家质检总局科技计划基金资助项目(2013IK-303);宁波市社会发展基金资助项目(2012C50049)

摘　　要：建立了洗涤用品中三氯生的高效液相色谱（HPLC）测定方法。以二氯甲烷为提取溶剂,采用超声提取法提取洗涤用品中的三氯生,用配有紫外检测器的高效液相色谱仪进行测定,流动相为V（甲醇）∶V（水）=90∶10,紫外检测波长为282 nm。当三氯生浓度在0.5~100 mg/L时,其HPLC峰面积与其浓度呈良好的线性关系。三氯生的检出限（3S/N）为0.1 mg/L,加标回收率在96.2%~104.3%之间,相对标准偏差为1.2%~4.4%。该方法准确、灵敏、快速、重复性好,能满足洗涤用品中三氯生定量分析的要求。The analytical method for the determination of triclosan in detergents was established with high performance liquid chromatography. Triclosan in detergents was ultrasonically extracted with dichloromethane,and determined by high performance liquid chromatography（ HPLC） equipped with ultraviolet（ UV） detector. Methanol-water（ 90∶ 10,V / V） was used as mobile phase at a flow rate of 1. 0 m L / min,and ultraviolet absorption wavelength was set at 282 nm for UV detector. Results showed good linear behavior in the triclosan concentration range from 0. 5 to 100 mg / L,with detection limit of（ 3S / N） 0. 1 mg / L. The spiked recoveries was 96. 2% ~ 104. 3% and the relative standard deviations of triclosan were in the range of 1. 2% ~ 4. 4%. The method established is sensitive,fast and simple with high accuracy and precision. It is can be used to determine triclosan in detergents.

关 键 词：洗涤用品 三氯生 高效液相色谱法 超声提取 

分 类 号：O657.7[理学—分析化学]