邻苯二甲酸二丁酯对大鼠卵巢颗粒细胞中细胞凋亡因子Bcl-2、Bax表达的影响  

Effect of dibutyl phthalate on the expression of apoptosis factor Bcl-2 and Bax in rat ovarian granulosa cells

作　　者：王雪金[1] 周云清[1] 罗湘闽[1] 刘瑾[2] 

机构地区：[1]福建医科大学附属第二医院妇产科,福建泉州362000 [2]福建医科大学公共卫生学院预防医学系

出　　处：《实用预防医学》2015年第2期155-158,共4页Practical Preventive Medicine

基　　金：泉州市科技项目(2011Z96);福建省卫生厅青年立项(2012-2-52)

摘　　要：目的研究邻苯二甲酸二丁酯(dibutyl phthalate,DBP)对大鼠卵巢颗粒细胞中细胞凋亡因子Bcl-2、Bax表达的影响。方法体外培养25 d雌性SD大鼠卵巢颗粒细胞,分别加入DBP使终浓度分别(0、5、20、80μmol/L),培养24 h。采用放射免疫方法测定雌二醇(estradiol,E2)和孕酮(progestone,P)的水平;采用实时荧光定量PCR(real-time PCR)和免疫印迹试验(Western blot)检测卵巢颗粒细胞中Bcl-2、Bax mRNA和蛋白的表达。结果 DBP染毒后颗粒细胞分泌的E2及P水平下降,各剂量DBP均可降低颗粒细胞中Bcl-2 mRNA和蛋白的表达(P<0.05),且表达量与DBP剂量呈反比,同时,DBP可以增加颗粒细胞中Bax mRNA和蛋白的表达(P<0.05),且表达量与DBP剂量呈正比。结论 DBP可导致大鼠卵巢颗粒细胞凋亡,且Bcl-2/Bax比例失调可能是导致其凋亡的机制。Objective To study the effect of dibutyl phthalate（ DBP） on the expression of apoptosis factors,B- cellymphoma- 2（ Bcl- 2） and Bcl- 2- associated X protein（ Bax） mRNAs in rat ovarian granulosa cells. Methods 25- day- old female Sprague- Dawley rats were injected with pregnant mare＇s serum gonadotropin（ PMSG）. The ovarian granulosa cells were collected and cultured in dulbecco＇s modified eagle medium（ DMEM）,and then exposed to different concentrations of DBP（ 0,5,20 and 80 μmol / L） for 24 h. Estradiol and progesterone in the ovarian granulosa cells were measured by the method of radio- immunity. The expression of Bcl- 2 and Bax mRNA and protein in the ovarian granulosa cells was examined by real- time PCR and Western blot,respectively. Results The levels of estradiol and progesterone in the ovarian granulosa cells decreased after being exposed to DBP. With the increase of the DBP concentrations,the expression of Bcl- 2 mRNA and protein decreased gradually（ P〈0. 05） and were inversely related to the DBP concentration,while the expression of Bax mRNA and protein increased gradually（ P〈0. 05） and were positively correlated with the DBP concentration. Conclusions DBP may induce the apoptosis of rat ovarian granulosa cells,and the disproportion between Bcl- 2 and Bax may be the mechanism of the apoptosis.

关 键 词：邻苯二甲酸二丁酯 颗粒细胞 雌二醇 孕酮 凋亡 

分 类 号：R711.59[医药卫生—妇产科学]