饮水消毒副产物DCN对HepG2细胞的脂质过氧化作用  

Effect of dichloroacetonitrile,a by-product of drinking water disinfection on lipid peroxidation of HepG2 cells

作　　者：罗皓[1,2] 杨慧[1,2] 陈佳佳[1,2] 杜进林[1] 

机构地区：[1]广东医学院公共卫生学院,广东东莞523808 [2]广东医学院环境与健康研究所

出　　处：《实用预防医学》2015年第2期162-164,共3页Practical Preventive Medicine

基　　金：湛江市科技攻关计划项目(2012C3101007);广东医学院博士启动项目(XB1309);广东医学院面上项目(XK1231)

摘　　要：目的研究饮水含氮消毒副产物二氯乙腈对体外培养的人肝肿瘤Hep G2细胞的脂质过氧化作用。方法二甲基亚砜(DMSO)溶解DCN,以DMSO处理组为溶剂对照组,设0.8、4、20、100μmol/L 4个暴露浓度,将Hep G2细胞分别染毒4和24 h后,检测Hep G2细胞内还原型谷胱甘肽(GSH)、丙二醛(MDA)、超氧化物歧化酶(SOD)的含量。结果在细胞染毒4 h的条件下,与溶剂对照组相比,DCN浓度达到100μmol/L时,Hep G2细胞内SOD含量明显下降(P<0.01),但GSH和MDA含量没有明显的变化;在染毒24 h条件下,与溶剂对照组相比,各染毒组的DCN使Hep G2细胞内GSH含量明显下降(P<0.05),100μmol/L的DCN可引起Hep G2细胞内MDA含量上升(P<0.05)和SOD含量下降(P<0.01)。相关分析表明,当染毒时间延长至24 h,MDA含量的变化与SOD含量的变化呈负相关(r=-0.64,P<0.05)。结论 DCN可导致Hep G2细胞氧化损伤,使其脂质过氧化反应增强、抗氧化作用降低。Objective To study the effect of dichloroacetonitrile（ DCN）,a by- product of drinking water disinfection on lipid peroxidation of human hepatocellular carcinoma cell line（ Hep G2） in vitro. Methods DCN was dissolved in dimethyl sulfoxide（ DMSO）,and Hep G2 cells treated with DMSO only were considered as solvent control group. Hep G2 cells were respectively exposed to DCN at the concentrations of 0. 8,4,20 and 100μmol / L for 4 and 24 h. The changes in the contents of glutathione（ GSH）,malondialdehyde（ MDA） and superoxide dismutase（ SOD） in Hep G2 cells were detected. Results As compared with solvent control group,the content of SOD was significantly decreased when Hep G2 cells were exposed to DCN at the concentration of 100μmol / L for 4 h（ P〈0. 01）,but no significant change was found in the contents of GSH and MDA. As compared with solvent control group,the content of GSH was significantly decreased（ P〈0. 05） when Hep G2 cells were exposed to DCN at the concentrations of 0. 8,4,20 and 100μmol / L for 24 h. At the concentration of 100μmol / L DCN,the content of MDA was increased（ P〈0. 05）,but the content of SOD decreased（ P〈0. 01） in comparison with solvent control group. The correlative analysis indicated that the changes of MDA content were negatively associated with the alteration of SOD（ r =- 0. 64,P〈0. 05） when Hep G2 cells were exposed to DCN for 24 h. Conclusions DCN may induce oxidative damage in Hep G2 cells,including the increased lipid peroxidation and the reduced antioxidation ability.

关 键 词：二氯乙腈 脂质过氧化 人肝肿瘤HepG2细胞 

分 类 号：R114[医药卫生—卫生毒理学]