高脂血症性急性胰腺炎大鼠模型的建立和分析  

Establishment of hyperlipidemic acute pancreatitis animal model in rats

作　　者：黄晓丽[1] 王国品[1] 王平[1] 滕晓琨[1] 葛建新[1] 

机构地区：[1]南通大学附属南京江北人民医院消化科,江苏南京210048

出　　处：《中国临床研究》2015年第2期148-150,共3页Chinese Journal of Clinical Research

基　　金：南通大学自然科学研究项目(10Z102)

摘　　要：目的探讨高脂血症性急性胰腺炎(HLP)大鼠模型的建立。方法 32只SD雄性大鼠随机分为4组:正常(N)组,高脂血症(HL)组,非高脂血症性急性胰腺炎(NHLP)组,HLP组,每组8只。N组和HL组分别予以普通饲料和高脂饲料喂养4周后,术中仅轻轻翻动十二指肠和胰腺;NHLP组和HLP组分别以普通饲料和高脂饲料喂养4周后,5%牛磺胆酸溶液逆行胰胆管注射制模。观察各组术后12 h血清淀粉酶(AMS)、甘油三酯(TG)及胰腺病理变化。结果血清TG水平HLP组[(0.64±0.25)mmol/L]明显高于NHLP组[(0.30±0.14)mmol/L,P<0.05]和N组[(0.16±0.03)mmol/L,P<0.01]。血清AMS水平NHLP组[(46.49±27.95)U/L]与HLP组[(60.29±49.53)U/L]明显高于N组[(6.12±2.00)U/L,P<0.01]。NHLP组与HLP组胰腺实质大量坏死伴出血,间质显著充血水肿,大量炎细胞浸润,病理组织学评分NHLP组(9.13±1.55)与HLP组(10.88±1.55)明显高于N组(0.25±0.46)(P均<0.01)。结论高脂饲料喂养联合逆行胰胆管注射制备HLP大鼠模型,具有稳定性高、成活率高等优势,是理想的HLP制模方法。Objective To establish the hyperlipidemic acute pancreatitis（ HLP） animal model in rats and observe the molding effect. Methods Thirty-two male SD rats were randomly divided into 4 groups（ n = 8 each） ： normal group（ N group）,hyperlipidemia group（ HL group）,non-HLP group（ NHLP group） and HLP group. In N group and HL group,the duodenum and pancreas were gently ruffled only in operation after fed with ordinary diet and high fat diet for 4 weeks,respectively. In NHLP group and HLP group,5% taurocholic acid solution was injected into the biliopancreatic duct by retrograde direction for molding after fed with ordinary diet and high fat diet for 4 weeks,respectively. The levels of serum amylase（ AMS） and triglyceride（ TG） and the pancreatic pathological changes in all groups were observed 12 hours after operation. Results The serum TG level in HLP group [（ 0. 64 ± 0. 25） mmol / L]were significantly higher than those in NHLP group[（ 0. 30 ± 0. 14） mmol / L,P〈0. 05] and N group [（ 0. 16 ± 0. 03） mmol / L,P〈0. 01]. The serum AMS level in NHLP group[（ 46. 49 ± 27. 95） U / L] and HLP group[（ 60. 29 ± 49. 53） U / L] were all significantly higher than that in N group [（ 6. 12 ± 2. 00） U / L,all P〈0. 01]. The massive necrosis and hemorrhage of pancreas parenchyma,obvious interstitial edema and a large number of inflammatory cells infiltration were found in NHLP group and HLP group. The histopathology scores in NHLP group（ 9. 13 ± 1. 55） and HLP group（ 10. 88 ± 1. 55） were all significantly higher than that in N group（ 0. 25 ± 0. 46）（ all P〈0. 01）. Conclusion The HLP rat model established by high fat diet combined with retrograde injecting 5% taurocholic acid solution into the biliopancreatic duct has the advantages of high stability and high survival rate,and is an ideal method for establishing model.

关 键 词：胰腺炎 高脂血症 动物模型 大鼠 

分 类 号：R332[医药卫生—人体生理学] R576[医药卫生—基础医学]