基于聚集诱导荧光性质的免标记DNA四链体检测以及凝血酶的传感研究  

A Label-free Fluorometeric Method for Screening the G-quadruplex and Thrombin Based on Aggregation-induced Emission

作　　者：汪铭[1] 张关心[1] 张德清[1] 

机构地区：[1]中国科学院化学研究所有机固体实验室,北京100190

出　　处：《影像科学与光化学》2015年第1期77-83,共7页Imaging Science and Photochemistry

摘　　要：基于带正电荷硅杂环戊二烯衍生物的聚集诱导荧光性质,利用其与富含G的单链DNA和四链体作用后的荧光强度差别,发展了一种免标记的DNA四链体检测方法,并将该方法应用于凝血酶的荧光分析。The positively chargedsilole derivative, 1-Methyl-1-（3-trimethylamino）propyl-2,3,4,5-tetraphenylsilole iodide,can differentiate single strand DNA andG-quadruplex by modulating its aggregation and fluorescence emissionwith the binding of DNA.A new label-free fluorescence method for G-quadruplex and thrombin detection was further established by making use of the aggregation-induced emission feature of the silole derivative.

关 键 词：聚集诱导荧光 荧光传感器 G-四联体 凝血酶 

分 类 号：O657.3[理学—分析化学] R440[理学—化学]