南海长棘海星共附生真菌的分离鉴定及多样性分析  

Isolation,Identification and Diversity of Fungi Associated with Acanthaster planci Collected from the South China Sea

作　　者：刘威[1] 梁婉玲[2] 谢中梁 蓝文健[2,3] 李厚金[4] 杨得坡[2,3] 王来友[1,5] 

机构地区：[1]广东药学院中医药研究院,广东广州510006 [2]中山大学药学院,广东广州510006 [3]广东省现代中药工程技术研究开发中心,广东广州510006 [4]中山大学化学与化学工程学院,广东广州510275 [5]广东省中医药防治代谢性疾病重点实验室,广东广州510006

出　　处：《中山大学学报（自然科学版）》2015年第1期84-88,95,共6页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(81102502;30973633);广东省科技计划资助项目(2012A031100005;2010B030600011);广东省自然科学基金资助项目(2011Y1-00036);中山大学高校基本科研业务费专项基金资助项目(11lgpy09)

摘　　要：长棘海星(Acanthaster planci)是热带和亚热带海域珊瑚礁的主要破坏者,体内外有丰富的共附生微生物。该文对采自南海三亚珊瑚礁保护区的一个长棘海星样品的共附生真菌,利用3种培养基进行分离纯化得到近200株真菌菌株。采用通用引物ITS1和ITS4扩增其中16株真菌的ITS-r DNA片段序列,并进行测序,测试结果提交Gen Bank,登录号为KF999808-KF999822以及KJ723458。利用邻接法构建系统发育树及简单分析该长棘海星共附生真菌的种群多样性。序列分析显示,16株真菌分别属于Ascomycota门4目(Hypocreales、Eurotiales、Pleosporales、Microascales),5属(Trichoderma、Penicillium、Neosartorya、Leptosphaerulina、Pseudallescheria),以及Deuteromycota门1目(Meliolales),1属(Fusarium,有性型Gibberella),并聚为8类。多样性分析的结果显示,菌体的个体数在镰刀属上(10株)表现为高度富集,群落的均匀度比较低。Acanthaster planci,commonly known as the crown of thorns starfish,usually preys upon scler-actinian corals.Recently,Acanthaster planci has been found biocenotic association with diverse and a-bundant microbial communities.Using three different media,nearly 200 strains of the endophytic fungi were isolated from the Acantaster planci collected from the Sanya,Hainan province,China.The ITS-rD-NA gene of 16 strains were sequenced,and submitted to GenBank.The register numbers of the se-quences for these strains are KF999808-KF999822 and KJ723458.The phylogenetic tree was then con-structed by using a neighbor-joining method,as well as the population diversity analysis.16 strains be-longing to 2 phyla （Deuteromycota,Ascomycota）,5 orders （Hypocreales,Eurotiales,Meliolales,Mi-croascales）,6 genera （Trichoderma,Pseudallescheria,Penicillium,Neosartorya,Leptosphaerulina, Pleosporale,Fusarium,）were classified into 8 groups.And ten strains belonging to Fusarium indicates that Fusarium is the dominant fungi,and the endophytic fungi community with form of commensalism of Acanthaster planci has the low degree of homogeneity.

关 键 词：长棘海星 共附生真菌 种群多样性 

分 类 号：Q939.5[生物学—微生物学]