MSAP在水稻害虫白背飞虱中的应用研究  

The Application of MSAP in Sogatella furcifera( Horvath)

作　　者：张梅[1] 陈佳林[1] 周晓穗[1] 梁士可 李广宏[1] 王方海[1] 

机构地区：[1]中山大学有害生物控制与资源利用国家重点实验室//昆虫学研究所,广东广州510275

出　　处：《中山大学学报（自然科学版）》2015年第1期98-102,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(31171844);广东省自然科学基金资助项目(S2011010001353;031628)

摘　　要：甲基化敏感的扩增多态性分析方法 (MSAP)是在扩增片段长度多态性技术基础上建立起来的主要用来检测样品基因组DNA甲基化情况的一种方法,特别适用于全基因组范围内检测CCGG位点的胞嘧啶甲基化情况,在高等动物和许多植物上已广泛应用,但尚未有在昆虫中应用该方法的报道。从DNA提取、酶切基因组DNA用量、酶切和接头连接时间、电泳图谱显影方法等方面对MSAP方法进行了改进,并在水稻重要害虫白背飞虱上得到成功应用。这为将来在其它昆虫中使用该方法研究基因组DNA甲基化情况提供了一定借鉴和指导价值。且通过比较白背飞虱雌雄性别间和长短翅型间的MSAP图谱,发现雌雄性别间和长短翅型间的基因组DNA甲基化式样都存在明显的差异,说明DNA甲基化可能参与了性别和翅型分化的调节。The methylation-sensitive amplification polymorphism （MSAP）technique is an amplified frag-ment length polymorphism （AFLP）based method for detection of DNA methylation.It represents a relia-ble technique for the detection of cytosine methylation alterations at the site of CCGG in a genome-wide perspective.MSAP has been extensively applied in plants and some animals,but no application in in-sects has been reported yet.The method was improved from DNA extraction,the amount of genomic DNA used for restriction enzyme digestion,time for enzyme digestions and ligation of adapters,and electropho-resis imaging method.And the method was applied successfully in Sogatella furcifera （Horvath）,an im-portant rice pest in China.It will provide some references and guiding values for the study of genome DNA methylation in other insects by using this method in the future.By comparing MSAP maps between male and female or between long wing and short wing of S.furcifera,it was found that there were obvious differences in DNA methylation patterns.This indicates that DNA methylation may be involved in gender and wings type differentiation.

关 键 词：DNA 甲基化 白背飞虱 性别分化 翅二型 

分 类 号：Q965[生物学—昆虫学]