梅毒螺旋体特异性抗体检测方法的实验室评价  

Evaluation of combined detection of antibodies specific for syphilis

作　　者：佘亚辉[1] 韩媛媛[1] 周光庭[1] 贾芳岩[1] 

机构地区：[1]亳州市人民医院检验科,安徽236800

出　　处：《国际检验医学杂志》2015年第3期324-325,共2页International Journal of Laboratory Medicine

摘　　要：目的对ELISA法联合化学发光法用于特异性梅毒抗体检测的效果进行评价。方法选取2014年1月至2014年4月于该院皮肤性病科就诊患者,共628例。对患者血清采用ELISA法进行初筛,再将检测结果在复检标准范围内(0.5<S/CO<3)的样本用化学发光法进行复检,然后与梅毒螺旋体明胶凝集试验(TPPA)法平行测定的结果进行比较。结果 TPPA法检测为阳性的标本为171例;ELISA法检测符合该科梅毒阳性判断标准的标本共162例,另外还有15例处于该科梅毒。经化学发光法进一步复检,阳性结果为9例,阴性结果为6例,并且此联合检测法得到的171例阳性结果与TPPA法阳性结果完全吻合。结论 ELISA法与化学发光法联合检测的结果与TPPA有很好的一致性。Objective To evaluate ELISA combined with chemiluminescence in the detection of syphilis specific antibodies.Methods 628 patients who came to our hospital and treated in dermatology department were recruited in the study from January2014 to April 2014.Patients′serum samples were detected by using ELISA firstly,and then the samples,the test rusults of which were within the recheck standard range（0.5〈S/CO〈3）were retested by using chemiluminescence.And then the results of parallel Treponema pallidum gelatin agglutination test（TPPA）measurement were compared with.Results There were 171 cases of TPPA positive samples detected and 162 cases meets the positive criteria for syphilis（S/CO≥3.0）in the hospital.In addition to that,15 cases of syphilis detected were within the recheck standard range.In the samples rechecked by using chemiluminescencea,there were9 cases of positive results,and 6cases of negative results.171 cases of positive results were detected by the combined method,which were consistent with TPPA.Conclusion The detection results of ELISA combined with chemiluminescence detection are consistent with that of TPPA.

关 键 词：化学发光法 酶联免疫吸附测定 梅毒螺旋体明胶凝集试验 

分 类 号：R446.6[医药卫生—诊断学] R759.1[医药卫生—临床医学]