菜豆组培快繁技术研究  

Study on Technology of Tissue Culture and Rapid Propagation in Common Bean(Phaseolus vulgaris L.)

作　　者：韩启厚[1] 古瑜[1] 于海龙[1] 李玉珍 刑堃 

机构地区：[1]天津科润蔬菜研究所,300380 [2]内蒙古通辽市开鲁县农业技术推广中心 [3]天津市北辰区种植业中心

出　　处：《长江蔬菜》2015年第2期50-53,共4页Journal of Changjiang Vegetables

基　　金：天津市农业科学院院长基金项目(编号:11017)

摘　　要：以双丰3号菜豆为试验材料,比较了种子消毒方法,筛选了快繁最佳增殖培养基、生根培养基及移栽基质。结果表明,25%次氯酸钠浸泡20 min对菜豆种子消毒效果最好;最佳增殖培养基为MSB5+6-BA 5.0 mg/L,14 d获得再生芽为9.2个/外植体;最佳生根培养基为1/2 MSB5+IBA 0.1 mg/L,生根率达95.3%;最佳移栽基质为草炭∶蘑菇渣=1∶1,移栽成活率为97%。In this paper, we took common bean cultivar Shuangfeng No.3 as experiment material, and compared seeddisinfection methods, in order to screen out rapid and suitable enrichment medium, rooting medium and transplantingmatrix. Tile results showed that, the disinfection effect of treatment with 25% sodium hypochlorite for soaking 20 minuteswas the best, and the most suitable enrichment medium was MSB5＋6-BA 5.0 mg/L, which could obtain 9.2 regeneratedbuds every explant after cultured 14 days. The most suitable medium for rooting was 1/2 MSB5＋IBA 0.1 mg/L, whichrooting rate was above 95.3%, while the most suitable transplanting matrix was peat：spent mushroom compost=1：1, withtransplanting survival rate of 97%.

关 键 词：菜豆 消毒 增殖 生根 移栽 

分 类 号：S643.1[农业科学—蔬菜学]