酶标法检测牛奶中硫氰酸盐  

Quantification of Thiocyanate in Milk with Microplate Reader

作　　者：侯东军[1] 韩合敬 吴艳芳 姜艳彬[1] 

机构地区：[1]中国动物疫病预防控制中心,北京100125 [2]青岛农业大学化学与药学学院,山东青岛266109 [3]菏泽职业学院应用化工系,山东菏泽274031

出　　处：《中国畜牧杂志》2015年第4期67-70,共4页Chinese Journal of Animal Science

基　　金：基金项目:农业部生鲜乳质量安全监测

摘　　要：本试验基于酶标仪法建立了用于快速、准确检测牛奶中硫氰酸根浓度值的方法,该方法用三氯乙酸除去牛奶中的蛋白和脂肪,过滤后取上清液,硫氰酸根与硝酸铁溶液显色后,用酶标仪在450 nm波长处测吸光度值,用模拟奶做标准曲线,通过科学的数据处理,定量测定牛奶中硫氰酸盐的含量。试验检测体系适用对象为生鲜乳及复原乳,试验结果表明：当硫氰酸根的浓度在0.5~16 mg/kg范围内时,硫氰酸根的质量浓度与溶液的吸光度值呈现良好的线性关系,相关系数为0.999 7,回收率为98.6%~105%,仪器检测下限为0.3 mg/kg。本试验所建立的方法数据可信度高,检测灵敏度高,成本低廉,操作方便,适合中小单位对牛奶中硫氰酸根做定量分析。In order to determine the concentrantion of SCN-in the milk, we have developed a method with the microplate reader . We cleared the protein and fat in the milk with trichloroacetic acid,based on the reaction between Fe3 ＋ and different concentration of SCN-, different absorbance value would occur. Determining the concentration of SCN- with the standard curve which was made with simulated milk was available. Result ：The results showed that the system could be used to determine raw milk and reconstituted milk .In addition ,the result showed that the concentration of SCN-between 0.5 mg/kg and 16 mg/kg formed a good Linearship with absorbance （R^2=0.999 7）. The recovery was between 98.6%-105%. Conclusion： The method was accurate,reliable and simple without using expensive devices.

关 键 词：硫氰酸盐 牛奶 酶标仪 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]