腺病毒潜伏感染加重生物燃料烟雾所致的豚鼠肺部及血清中氧化-抗氧化失衡  

Effects of adenovirus 5 latent infection on pulmonary oxidant-antioxidant imbalance caused by biomass fumes in guinea pigs

作　　者：石喆[1] 胡国平[1] 李冰[2] 冉丕鑫[1] 

机构地区：[1]广州医科大学第一附属医院广州呼吸疾病研究所呼吸疾病国家重点实验室,510120 [2]广州医科大学第一附属医院 广州呼吸疾病研究所 实验医学研究中心,510120

出　　处：《国际呼吸杂志》2015年第2期81-86,共6页International Journal of Respiration

基　　金：广东省自然科学基金研究团队资助项目（05200239）

摘　　要：目的探讨腺病毒潜伏感染在生物燃料烟雾所致的豚鼠肺部及血清中氧化-抗氧化失衡中的作用。方法将46只白化豚鼠用随机数字表法分为感染组（26只）和假感染组（20只），感染组鼻腔接种野生型腺病毒5型，假感染组接种PBS作为对照。22d后随机将各组剩余动物（各20只）再各分为暴露于木材锯末和谷壳烟雾组和暴露于新鲜空气的对照组。暴露结束后进行肺组织病理检测，并检测肺组织匀浆及豚鼠血清中的谷胱甘肽过氧化物酶（GSH—PX）、还原型谷胱甘肽（GSH）、丙二醛（MDA）、超氧化物歧化酶（s0D）、过氧化氢酶（cAT）、总抗氧化能力及BALF中的MDA、SOD、总抗氧化能力。所有数据均以互±8表示，采用SPSS15．0软件进行析因方差分析，组间比较采用单因素方差分析，P〈0．05为差异有统计学意义。结果腺病毒潜伏感染使豚鼠肺组织匀浆及血清中GSH—PX、GSH、SOD、总抗氧化能力显著降低（F值分别为171．024、109．05、24．704、41．660、23．513、12．652、12．447、14．912，P值均〈0．01），使豚鼠肺组织匀浆cAT降低（F=5．081，P〈0．05），MDA升高（F=5．725，P〈0．05）。生物燃料烟雾暴露使豚鼠肺组织匀浆及血清中GSH—PX、GSH、SOD、CAT、总抗氧化能力显著降低（F值分别为316．979、430．92、36．462、164．64、175．621、109．86、271．558、16．710、94．802、56．668，P值均〈O．01），MDA显著升高（F值分别为56．083、32．928，P值均〈O．01）。腺病毒感染和生物燃料烟雾暴露的交互效应使豚鼠肺组织匀浆GSH—PX降低（F=4．384，P〈O．05）及血清中GSH—PX、GSH降低（F=13．233，P〈O．01；F=5．979，P〈O．05），腺病毒感染＋生物燃料烟雾暴露组与单纯生物燃料烟雾暴露组比较，豚鼠肺组织匀浆中GSH、SOD、CAT、总抗氧化能力降低（F值分别为19．690、17．177、14．630、16．070，P值均Objective To investigate the effects of adenovirus 5 latent infection on the oxidant- antioxidant imbalance caused by exposure to biomass fumes in guinea pigs. Methods Forty-six albino guinea pigs were randomly divided into two groups. One group （ n = 26） was infected intranasally with adenovirus 5, and the other group （ n = 20） was shame-infected with sterile PBS as control. After 22 days, the living animals （20 each group） were randomly divided into two subgroups. One subgroup was exposed to biomass fumes for three weeks, and the other subgroup was exposed to room air. At the end of the experiment,animals were sacrificed and lung tissues were examined with histopathology. The persistence of the adenovirus latent infection was confirmed at the end of the study by immunohistochemistry to demonstrate the adenovirus EIA protein in the lungs of infected animals. The activities of glutathione peroxidase （GSH-PX）, superoxide dismutase （SOD）, and catalase （CAT）, the levels of glutathione （GSH） and malondialdehyde （MDA）, total ability of antioxidant in lung tissue homogenate and serum were measured. The activity of SOD, MDA and total ability of antioxidant in the bronchoalveolar lavage fluid were measured. Results The latent infection of adenovirus was associated with significant decrease of GSH-PX, GSH, SOD, total ability of antioxidant in the lung tissue homogenate and serum. However, it caused decrease of CAT and increase of MDA in lung tissue homogenate. At the same time, the biomass fumes was associated with obvious decrease of GSH-PX, GSH, SOD, CAT, total ability of antioxidant in the lung tissue homogenate and serum. It was demonstrated that the biomass fumes and the latent infection of adenovirus were associated with significant decrease of GSH-PX in lung tissue homogenate and GSH PX,GSH in serum. Conclusions Biomass fumes can lead to the oxidant-antioxidant imbalance in the lung tissue of guinea pigs,and the imbalance is amplified by the latent infection of adenovirus 5.

关 键 词：烟雾吸入损伤 腺病毒科感染 氧化-抗氧化失衡 豚鼠 

分 类 号：R563.1[医药卫生—呼吸系统]