过敏性支气管哮喘外周血中调节性T细胞表达IL-4的水平及临床意义  

The expression of IL-4 of regulatory T cells in peripheral blood of patients with allergic asthma and its clinical significance

作　　者：王林林[1,2] 时国朝[1] 万欢英[1] 周敏[1] 侯小霞[1] 潘丽娜[1] 陶连琴[1] 石宝玉[1] 宋慧慧[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院呼吸科,200025 [2]第二军医大学附属上海长征医院呼吸科,200003

出　　处：《国际呼吸杂志》2015年第3期161-164,共4页International Journal of Respiration

基　　金：上海自然科学基金基础重点研究项目（08JCl415200）;国家自然科学基金（81270083）

摘　　要：目的调节性T细胞（Treg）调节机体免疫平衡和免疫耐受。Treg细胞具有可塑性，可分泌炎性因子。但在过敏性支气管哮喘（简称哮喘）中，对于Treg细胞是否分泌IL-4及临床意义尚不清楚。故本研究主要探讨Treg细胞表达IL-4的特征及临床意义。方法本研究纳入对屋尘螨过敏的哮喘患者作为研究对象，健康志愿者作为正常对照组。采用流式细胞术检测外周血Treg细胞表达IL-4水平。结果正常对照组（n=20）、间歇轻度组（n=14）、中重度组（n=13）、IL-4＋ Treg／Treg％分别为0．72（O．35～1．02）％，2．46（1．15～3．56）％，5．60（2．10～10．21）％，间歇轻度组较正常对照组升高，差异有统计学意义（P〈0．05），中重度组较间歇轻度组升高，差异有统计学意义（Pd0．01）。结论过敏性哮喘患者外周血中Treg细胞可分泌IL-4，与疾病严重度密切相关，故推测IL-4＋ Treg细胞在过敏性哮喘炎症反应过程中起重要作用。Objective Regulatory T （Treg） cells were discovered as immune suppressors critical for self-tolerance and immune homeostasis. Treg cells were recently shown to have the plasticity, and secret inflammatory cytokines. However,whether do Treg cells secret IL-4 and its clinical significance in patients with allergic asthma remain unclear. In our study,we investigated the expression of IL-4 by Treg cells and its clinical significance. Methods We analyzed T cells in the peripheral blood of patients with allergic asthma and healthy volunteers. The frequencey of IL-4＋ Treg in peripheral blood was determined by flow cytometry. Results Our data showed that： （1）The percentage of IL-4＋ Treg/Treg in peripheral blood with intermittent-mild asthma （ n = 14） was higher than that in healthy controls（ n = 20）[2.46 （1.15-3.56）% vs 0. 72（0. 35-1. 02）%, P 〈0. 051. OThe percentage of IL-4＋ Treg/Treg in peripheral blood with moderate-severe asthma （ n = 13） was higher than that intermittent-mild asthma （ n = 14） [5.60（2.10-10.21）% vs 2. 46 （1.15-3. 56）% , P 〈0.01]. Conclusions Treg cells in peripheral blood could secret IL-4,and closely related to the severity of asthma. Thus, we presumed that IL-4＋ Treg cells play an important role in mediating inflammatory response.

关 键 词：过敏性哮喘 调节性T细胞 白介素4 

分 类 号：R562.25[医药卫生—呼吸系统]