胃炎口服液对Hp感染小鼠血清及胃黏膜细胞因子的影响  

Effect of Gastritis Oral Liquid on serum and gastric mucosal cytokines of mice with Hp infection

作　　者：李莉[1] 李卫红[2] 江恒[1] 敬新蓉[1] 卢海波[1] 冯怀志[1] 孙薏[1] 

机构地区：[1]解放军 452 医院肿瘤科,成都610021 [2]四川省军区后勤部卫生所

出　　处：《西南国防医药》2015年第2期129-131,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：成都军区十二五重点项目(B12011)

摘　　要：目的研究胃炎口服液对幽门螺杆菌(Hp)感染小鼠血清IL-2、IL-8及胃黏膜IL-8和IFN-γ的影响,探讨胃炎口服液治疗幽门螺杆菌相关性胃炎(HAG)的机制。方法实验小鼠被随机分为4组:正常对照组(D组)、模型对照组(C组)、丽珠维三联组(阳性对照,B组)和胃炎口服液组(A组),每组15只。采用Hp菌液灌胃法制备Hp感染小鼠模型,D组用正常小鼠,后3组用模型小鼠进行实验。前两组给予等体积的生理盐水灌胃,后两组分别用丽珠维三联和胃炎口服液灌胃,均灌胃2 w。2 w后处死动物,观察各组小鼠胃黏膜病理学改变,ELISA检测血清IL-2和IL-8的水平以及胃黏膜IL-8和IFN-γ的含量。结果与C组比较,胃炎口服液(A组)能显著减轻HAG小鼠胃黏膜炎症,降低血清和胃黏膜IL-8及胃黏膜IFN-γ的含量(P<0.05),并提高血清IL-2的水平(P<0.05),但其作用尚不及丽珠维三联(B组)明显。结论胃炎口服液能调节IL-2和IL-8、IFN-γ之间的失调,进而对HAG有治疗作用。Objective To study the effect of Gastritis Oral Liquid on serum 1L-2 and IL-8 as well as gastric mucosal cytokines IL-8 and IFN--γ of mice with Helicobacter pylori（Hp） infeetion,and explore the mechanism of Gastritis Oral Liquid in the treatment of Helicobacter pylori-associated gastritis（HAG）. Methods Kunming mice models infected by human Hp were built by intragastric lip administration. All the mice were randomly divided into 4 groups ： normal control group （ group D ）, model control group （ group C ）, Lizhuwei Sanlian group （positive group, group B ） , and Gastritis Oral Liquid group （group A;n = 15 per group）. They were treated with： group A ： Gastritis Oral Liquid; group B ： Lizhuwei Sanlian; group C ： normal saline; group D ： normal saline. The treatments were carried out once a day for 2 weeks for each group, and then these mice were killed in order to observe the pathological changes of the mice＇s gastric mucosa and detect the serum levels of IL-2 and IL-8 and contents of gastric mucosal cytokines IL-8 and IFN-γ by ELISA. Results Compared with group C,Gastritis Oral Liquid reduced significantly the HAG mice gastric mueosa inflammation, reduced the serum levels of IL-8 and contents of gastric mueosal eytokines IL-8 and IFN-γ（ P 〈 O. 05 ）, and increased the serum levels of IL-2 （ P 〈O. 05）. But the effects of Gastritis Oral Liquid was less than that of Lizhuwei Sanlian. Conclusion Gastritis Oral Liquid may play a protective effect on the gastric mucosa in HAG mice through adjusting the balance of cytokine network, maintaining the balance between the anti-inflammatory cytokines （IL-2） and pro-inflammatory cytokines （ IL-8 and INF-γ ）.

关 键 词：幽门螺杆菌 小鼠 胃炎口服液 IL-2 IL-8 IFN-Γ 

分 类 号：R285.6[医药卫生—中药学]