基于罗丹明B新型Fe^(3+)荧光探针的合成及其性能研究  

Synthesis and Properties Characterization of Novel Fe^(3+) Fluorescent Probe Based on Rhodamine B

作　　者：苏娜[1] 杨美盼[1] 孟文斐[1] 杨秉勤[1] 

机构地区：[1]合成与天然功能分子化学教育部重点实验室西北大学化学与材料科学学院,西安710069

出　　处：《有机化学》2015年第1期175-180,共6页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.21172178)资助项目~~

摘　　要：以罗丹明B与2,6-二氨基吡啶缩合反应得到新型罗丹明B衍生物R1,其结构经1H NMR,13C NMR,IR,HRMS-ESI,X-Ray单晶衍射及元素分析表征.研究发现化合物R1作为荧光探针能选择性比色地识别Fe3+,且受常见金属离子的干扰较小.探针R1的荧光强度与Fe3+浓度(4×10-6~1.6×10-5 mol/L)呈较好的线性关系,并在Hep G2活细胞中实现了Fe3+的成像.A novel rhodamine B derivative R1 was synthesized facilely by the condensation reaction of rhodamine B and 2,6-diaminopyridine, and its structure was characterized by 1^H NMR, 13^C NMR, IR, HRMS-ESI, X-ray techniques and ele- mental analysis. The results showed that the compound R1 exhibited the selective colorimetric to Fe^3＋, and other metal ions did not interfere with its recognition. The fluorescence intensity of probe R1 and Fe3＋ concentration （4 ×10^-6- 1.6×10^-5 mol/L） showed a good linear relationship. What＇s more, probe R1 has been applied to image intracellular Fe^3＋ in living HepG2 cells.

关 键 词：罗丹明B 荧光探针 铁离子 识别 荧光强度 HepG2活细胞 

分 类 号：O621.3[理学—有机化学]