宁夏马铃薯Y病毒(PVY)外壳蛋白基因分子变异分析  

Genetic Variation of Coating Protein of Potato Virus Y in Ningxia Province

作　　者：陈晓军[1] 樊云芳[2] 王敬东[1] 张丽[1] 石磊[1] 宋玉霞[1] 

机构地区：[1]宁夏农林科学院农业生物技术研究中心/宁夏农业生物技术重点实验室,银川750002 [2]宁夏农林科学院枸杞工程技术研究中心,银川750002

出　　处：《湖北农业科学》2014年第22期5558-5561,共4页Hubei Agricultural Sciences

基　　金：国家科技支撑计划项目(2009BADC5B04)

摘　　要：马铃薯(Solanum tuberosum)Y病毒(Potato virus Y,PVY)是危害我国马铃薯的主要病毒之一。利用马铃薯Y病毒通用ELISA试剂盒确定所携带Y病毒的试验材料,设计了特异性引物通过反转录PCR获得PVY CP基因序列,通过克隆PVY衣壳蛋白基因(Coat protein,CP)序列分析其进化与变异类型。结果表明,分离得到11株病毒,依据其CP序列特性分为4类;宁夏地区主要流行的病毒类型为PVYO进化分枝,同时也可能出现了具有高致病性的PVYNTN新株系,占到被检出病毒分离株的9.1%。结果揭示了宁夏地区PVY的进化与变异类型,有助于针对性地防控PVY。Potato（Solanum tuberosum） virus Y is a harmful virus for potato industry. The plant materials with PVY were determined with general PVY ELSIA kit. The sequences of PVY coat protein were obtained by RT-PCR. Evolution and variation were analyzed by bioinformatics software. The results showed that sequences of 11 PVY coat protein were obtained and divided into four virus types. The main virus type was PVY O clade in Ningxia areas. Highly pathogenic virus type PVYNTN were detected, accounting for 9.1%. It will help pertinently prevent PVY, and understand the virus type and evolution of PVY in these areas.

关 键 词：马铃薯(Solanum tuberosum) 马铃薯Y病毒 分子变异 

分 类 号：S532[农业科学—作物学]