东方蜜蜂卵黄原蛋白基因cDNA克隆及其基本生物信息学特征  

Cloning and fundamental bioinformatical characteristics of Vitellogenin cDNA in honeybees Apis cerana

作　　者：王秀秀[1] 杨明华[1,2] 李昌[3] 匡海鸥[3] 和绍禹[3] 李亚辉[3] 

机构地区：[1]云南农业大学动物科学技术学院,昆明650201 [2]云南农业大学云南省动物营养与饲料重点实验室,昆明650201 [3]云南农业大学食品科学技术学院,昆明650201

出　　处：《中国蜂业》2015年第3期12-15,共4页Apiculture of China

基　　金：现代农业(蜜蜂)产业技术体系建设专项资金资助(CARS-45-kxj14)

摘　　要：卵黄原蛋白(vitellogenin,Vg)基因在蜜蜂的卵巢发育、胚胎营养、劳动分工、行为调控、气候适应、寿命延长等方面起着重要作用,但东方蜜蜂中该基因的基础数据却鲜有报道。本研究对东方蜜蜂Vg基因的cDNA全长序列进行了克隆、测序和基本的生物信息学分析,结果表明:东方蜜蜂Vg基因cDNA全长序列为5313bp,提交到Gen Bank的登录号为KP119837,该基因共编码1770个氨基酸,其cDNA和所编码的氨基酸序列与意大利蜜蜂的同源性分别为93%和89%,其保守区序列占全序列的63%。基于Vg基因对蜜蜂属内序列已知的4种蜜蜂构建的系统发育树显示:东方蜜蜂与意大利蜜蜂的亲缘关系最近,与大蜜蜂的亲缘关系稍远,与小蜜蜂的亲缘关系最远。本论文为进一步研究东方蜜蜂Vg基因的功能奠定了基础。Vitellogenin （Vg） gene plays a pivotal role in ovarian development, embryotrophy, labor division, behavior regulation, climate adaptation and life extending. However, the fundamental data of Vg gene in Eastern honeybees （Apis eerana） has rarely been reported. In the present study, the full-length eDNA of Vg in Apis cerana was cloned, sequenced and analyzed bioinformatically. The results indicate that： the full-length eDNA of Vg in Apis cerana was composed of 5313bp which encode 1770 amino acids. The Vg eDNA sequence of Apis cerana was presented to GenBank with the accession number of KP119837. The sequence identity of the Vg eDNA and the encoding amino acid for Apis cerana and Apis mellifera were 93% and 89%, respectively. The conserved eDNA sequence accounted for 63% in the whole sequence. Phylogenetic tree of Vg was constructed with 4 honeybee species in Apis genus whose Vg sequences are known. The genetic relationship analysis showed that Apis cerana is genetically closest to Apis mellifera, while it is further and furthest to Apis dorsata and Apis florea, respectively. This study will lay a foundation for the further study of Vg function in Apis cerana.

关 键 词：东方蜜蜂 卵黄原蛋白 Vg CDNA 克隆 生物信息学 

分 类 号：S891[农业科学—特种经济动物饲养]