树番茄高频再生体系的建立  

Establishing High-Frequency Regeneration System of Cyphomandra betacea

作　　者：植爽[1] 吴钰祥[1] 王玉杰[1] 王辉[1,2] 黄作喜[1,2] 

机构地区：[1]内江师范学院生命科学学院,四川内江641100 [2]四川省高等学校特色农业资源研究与利用重点实验室,四川内江641100

出　　处：《湖北农业科学》2014年第23期5880-5883,共4页Hubei Agricultural Sciences

基　　金：国家级大学生创新创业训练计划项目(201310640010;内江师范学院创新创业训练计划项目(X201310)

摘　　要：以树番茄(Cyphomandra betacea)种子为材料,研究不同浓度的6-BA、NAA、IAA及IBA对树番茄愈伤组织及不定芽诱导、不定芽增殖及生根的影响,以建立一套最适的高频再生体系。结果表明,不定芽诱导的最适培养基是MS+0.20 mg/L 6-BA+30 g/L蔗糖;不定芽增殖的最适培养基是MS+2.0 mg/L 6-BA+0.1 mg/L NAA+30 g/L蔗糖;不定根诱导的最适培养基是1/2MS+0.1 mg/L IBA+30 g/L蔗糖。To establish a high-frequency regeneration system, the effects of different concentrations of 6-BA, NAA, IAA and IBA on the induction of callus and adventitious buds, proliferation and rooting were studied by using Cyphomandra betacea seeds. The results showed that the optimal medium for induction of adventitious buds was MS＋0.20 mg/L 6-BA＋30 mg/L sucrose. MS＋2.0 mg/L 6-BA＋0.1 mg/L NAA＋30 mg/L sucrose was optimal for proliferation of adventitious buds. The optimal medium for rooting was 1/2MS＋0.1 mg/L IBA ＋30 mg/L sucrose.

关 键 词：树番茄(Cyphomandra betacea) 高频再生体系 组织培养 

分 类 号：S641.9[农业科学—蔬菜学]