鲜地黄及其炮制加工品中维生素C的含量测定  

Determination the Content of Vitamin C in Fresh Rehmannia Root and Processed Products

作　　者：贾秀梅[1] 张振凌[1] 寇真真[1] 王瑞升[1] 

机构地区：[1]河南中医学院,河南郑州450046

出　　处：《中医学报》2015年第1期79-82,共4页Acta Chinese Medicine

基　　金：国家科技支撑计划项目(2011BAI06B02)

摘　　要：目的:优选维生素C的最佳提取工艺,并测定鲜地黄Rehmannia glutinosa Libosch及其炮制加工品中维生素C的含量。方法:用紫外分光光度法测定维生素C含量,并通过正交试验设计,以维生素C含量为指标,以提取溶剂、料液比、提取时间为影响因素,筛选出维生素C的最佳提取工艺。结果:通过正交试验分析后,得出超声波辅助提取鲜地黄及其炮制加工品中维生素C的最优工艺条件为:新沸并冷却后的双蒸水为提取溶剂,料液比为30∶1,超声提取时间45min。结论:提取溶剂对鲜地黄及其炮制加工品中维生素C的含量有显著性影响,鲜地黄不同炮制加工品中维生素C含量差别较大,均低于鲜地黄中维生素C的含量。Objective： To select the best extraction of vitamin C,and to determine its content in Shengdihuang（ Fresh Rehmannia Root）and processed products. Methods： By ultraviolet spectrophotometry determine vitamin C content and using the orthogonal experiment method to select the best extraction,extract solvent,dosage,extract time as factors. Results： Test indicated the best extraction method：new boiled puried water as extraction solvent,and material liquid ratio 30： 1,ultrasonic extraction time of 45 min. Conclusion： The extraction solvent have significant effect for the content. The content of vitamin C in fresh rehmannia root and processed products have great different,and the content in all processed products is lower than fresh rehmannia root.

关 键 词：鲜地黄 维生素C 紫外分光光度法 提取工艺 怀地黄 

分 类 号：R284.2[医药卫生—中药学]