芪仙清鸣颗粒对支气管哮喘小鼠气道炎性反应的作用及其机制  

Effect of Qixianqingming granule on airway inflammation in a mouse model of asthma

作　　者：赖一鸣[1] 倪振华[1] 李钊[1] 王雄彪[1] 

机构地区：[1]上海中医药大学附属普陀医院呼吸科,上海200062

出　　处：《上海医学》2014年第12期1037-1040,I0001,共5页Shanghai Medical Journal

基　　金：上海市科学技术委员会2012年科技攻关项目(12401900404);上海市科学技术委员会引领计划(10411969100);上海市教育委员会科研创新项目(13ZZ096);“085”一流学科建设科技创新支撑计划(085ZY1216)资助

摘　　要：目的通过小鼠支气管哮喘模型,探讨芪仙清鸣颗粒治疗支气管哮喘的作用及其机制。方法选取30只健康雌性BALB/c小鼠,随机分为空白对照组(对照组)、支气管哮喘模型组(哮喘组)和芪仙清鸣颗粒治疗组(治疗组)。采用卵白蛋白致敏法建立支气管哮喘小鼠模型,造模成功后予药物处理2周;采用苏木精-伊红(H-E)染色观察病理组织学变化,过碘酸雪夫(PAS)染色检测气道黏液分泌情况,酶联免疫吸附试验(ELISA)检测血清白细胞介素(IL)-4水平,免疫组织化学法检测人第10号染色体缺失的磷酸酶和张力蛋白同源的基因(PTEN)蛋白表达情况。PTEN蛋白表达以其阳性区域的面积(Area)/累积光密度值(IOD值)表示。结果对照组小鼠肺组织小支气管无炎性细胞浸润、气道无增厚,气道无明显黏液分泌;哮喘组小鼠肺组织呈明显炎性浸润、气道明显增厚,气道黏液分泌显著增加;治疗组小鼠肺组织炎性浸润较哮喘组缓解,气道略有增厚,气道黏液分泌明显减少。哮喘组小鼠的血清IL-4水平为(6.34±4.71)pg/mL,显著高于对照组的(2.28±1.10)pg/mL(P=0.016);治疗组小鼠的血清IL-4水平为(2.82±1.27)pg/mL,显著低于哮喘组(P=0.035)。哮喘组小鼠肺组织PTEN的表达量为(0.06±0.04)IOD/Area,显著低于对照组的(0.15±0.10)IOD/Area(P=0.011);治疗组小鼠肺组织PTEN的表达量为(0.11±0.04)IOD/Area,显著高于哮喘组(P=0.013)。结论芪仙清鸣颗粒能显著抑制支气管哮喘小鼠气道炎性反应,改善病理损伤。选择性上调PTEN可能是其治疗支气管哮喘的作用机制之一。Objective To explore the effect and mechanism of Qixianqingming granule for the treatment of bronchial asthma in mice. Methods Thirty healthy female BALB/c mice were randomly divided into three groups： normal control group, asthma group and Qixianqingming granule group. Asthma was induced by ovalbumin challenge in mice. Then Qixianqingming granule was given for two weeks in the Qixianqingrning granule group. Hematoxylin-eosin （H-E） staining was applied to observe the pathological changes. Periodic acid Schiff （PAS） staining was used to detect airway mucus secretion. Enzyme linked immunosorbent assay （ELISA） detection was used to detect the concentration of serum interleukin 4 （IL-4）. Immunohistochemistry was used to detect the expression of phosphatase and tensin homolog deleted on chromosome 10 （PTEN） protein. Results In the control group, no airway inflammation or mucus hypersecretion were found. In the asthma group, airway inflammation and mucus hypersecretion were significantly increased. But the airway inflammation was relieved and mucus hypersecretion was significantly decreased after applying Qixianqingming granule. The concentration of serum IL-4 in the astWna groN3 was significantly higher than that in the control group （[6.34 4±4.71[ pg/mL vs. [2.284±1. 10] pg/mL, P=0.016）. The concentration of serum IL-4 in Qixianqincj-ning granule group was （2.82 ± 1.27） pg/mL, which was significantly lower than that in the asthma group （ P = 0. 035）. The expression of PTEN in the asthma group was significantly lower than that in the control group （[0.06 ± 0.04] IOD/Area vs. [0.15±0.10] IOD/Area, P = 0.011 ）. The expression of PTEN in the Qixianclingrning granule group was （0. 11±0.04）IOD/Area, which was significantly higher than that in the asthma group （P = 0. 013）. Conclusion Qixianqingming granule can significantly inhibit airway inflammation and improve pathological injury of asthma. Selective upregulation of PTEN may be one of the mechanisms of Qixianqingming g

关 键 词：哮喘 芪仙清鸣颗粒 人第10号染色体缺失的磷酸酶和张力蛋白同源的基因 

分 类 号：R285.5[医药卫生—中药学]