Ⅰ型鸭肝炎病毒VP1基因克隆与序列分析  

Cloning and Sequence Analysis of VP1 Gene of Duck Hepatitis A

作　　者：任邵娜[1,2,3,4] 袁生[2] 蒲文珺[2] 彭巧丽[3,4] 王辉[3,4] 陈志伟[3,4,5] 张浩吉[1,2] 李国清[1] 

机构地区：[1]华南农业大学兽医学院,广州510642 [2]佛山科学技术学院动物医学系,广东佛山528231 [3]香港大学李嘉诚医学院艾滋病研究所深圳研究室 [4]深圳市第三人民医院,广东深圳518112 [5]香港大学李嘉诚医学院艾滋病研究所

出　　处：《湖北农业科学》2014年第21期5208-5212,5216,共6页Hubei Agricultural Sciences

摘　　要：为了了解鸭肝炎病毒的遗传变异情况,试验采用RT-PCR方法对采自广东省各地12个DHV临床分离株的VP1基因进行了扩增、克隆、测序和序列分析.结果表明,12个DHV分离株的VP1基因序列长度均为714 bp,编码238个氨基酸,12个分离株与GenBank公布的参考株核苷酸同源性为91.7％～95.8％,氨基酸同源性为94.5％～98.3％,而12个分离株之间的核苷酸序列同源性达到99.2％～100％,氨基酸同源性为97.9％～100％.系统分析证明,12个DHV分离株均为DHV-Ⅰ型,不同分离株VP1基因无碱基插入和缺失,但存在点突变,各DHV分离株间亲缘关系较近,但存在一定的遗传差异.In order to investigate the variation of Duck hepatitis virus （DHV）,a total of 12 DHV VP1 genes from different regions of Guangdong province in China were amplified,cloned and sequenced.The results showed that the lengths of all VP1 sequences were 714 bp,encoding 238 amino acids.Homology analysis revealed that the VP1 genes of 12 isolates shared 91.7％-95.8％ nucleotide identity and 94.5％-98.3％ amino acids identity with the reference strain of DHV from GenBank.Nucleotide sequence homology of VP1 gene among the 12 DHV isolates varied from 99.2％ to 100％,while amino acid sequence homology varied from 97.9％ to 100％.The phylogenetic analysis revealed that all the 12 isolates were DHV-Ⅰ,with no InDel,but having point mutations.All 12 isolates had a close genetic relationship with a few genetic differences.

关 键 词：Ⅰ型鸭肝炎病毒 VP1基因 克隆 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]